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| Publication Type:
| Report |
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| Author(s): | Wang, Juan |
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| Author Affiliation: | School of Life Sciences, Ningxia University, Yinchuan, Ningxia |
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| Title: | Optimization of conditions agrobacterium-mediated transformation in Kentucky bluegrass |
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| Source: | [Anhui Nongye Kexue Shuangyuekan] [Journal of Anhui Agricultural Sciences]. Vol. 1, 2010, p. Unknown. |
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| Publishing Information: | [China: Hefei] |
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| # of Pages: | 0 |
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| Related Web URL: | http://en.cnki.com.cn/Article_en/CJFDTotal-AHNY201001026.htm
Last checked: 04/25/2016
Notes: Abstract only |
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| Keywords: | TIC Keywords: Agrobacterium tumefaciens; Bud development; Cultivar evaluation; Poa pratensis
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| Abstract/Contents: | "[Objective] The study aimed to enhance the transformation frequency of Kentucky bluegrass. [Method] With shoot tips of multiple bud multiple as transformation target, the factors which influenced transformation of Kentucky bluegrass mediated by Agrobacterium tumefaciens were studied. [Result] Higher percentage of resistant buds (hygromycin-resistant buds / infected buds) was obtained under the following conditions: shoot tips were infected in Agrobacterium suspension of OD600=0.6 supplemented with 0.1 mmol/L acetosyrigone for 5 minites under 50 kPa pressure,and the highest percentage of resistant buds reached 15.19%. [Conclusion] The transformation efficiency was affected by the concentration of Agrobacterium suspension, vacuum infiltration and acetosyringone(AS) induction." |
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| Language: | English |
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| References: | Unknown |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, J. 2010. Optimization of conditions agrobacterium-mediated transformation in Kentucky bluegrass. [Anhui Nongye Kexue Shuangyuekan] [Journal of Anhui Agricultural Sciences]. 1:p. Unknown. |
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