Full TGIF Record # 271880
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http://cykx.lzu.edu.cn//article/2016/1001-0629-33-1-46.html
    Last checked: 05/20/2016
Publication Type:
i
Report
Author(s):Lei, Ya-hong; Kuang, Wei-gang; Zheng, Chun-sheng; Wang, Wan-chun; Li, Chun-jie; Gao, Wen-na
Author Affiliation:Lei and Li: Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou; Kuang: Department of Plant Pathology/Key Laboratory of Plant Pathology, China Agricultural University; Zheng, Wang, and Gao: Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, China
Title:Detection and identification of seed-borne fungi isolated from imported grass seeds
Source:[Caoye Kexue] [Pratacultural Science]. Vol. 33, No. 1, January 15 2016, p. 46-53.
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Ke Xue" Bian Jibu]
# of Pages:8
Keywords:TIC Keywords: Alternaria; Aspergillus; Disease identification; Festuca arundinacea; Fusarium; Identification keys; Isolation technique; Lolium perenne; Seed testing; Seedborne fungi; Sorghum sudanense; Strain
Abstract/Contents:"The 7 grass seed samples imported from 5 countries were analyzed for seed-borne fungi by Petri-dish testing. The samples included perennial ryegrass (Lolium perenne), tall fescue (Festuca arundinacea) and sudan grass (Sorghum sudanense) in 2014. The ITS (Internal transcribed spacer) segment was amplified using ITS4 and ITS5 as primers, then purified and sequenced. The ITS sequences obtained were blasted with known ITS sequences in database. The results showed that 48 fungal strains belonged to 21 species of 13 genera were isolated, and the seed-borne fungal species of seed samples were significantly different from each other. The fungal species were mainly Fusarium spp. Aspergillus spp., Phoma spp., and Alternaria spp.. According to the ITS phylogenetic tree, all fungi could be identified to genus, which were hard to species. Alternaria tenuissima, A. alternate, and A. arborescens couldn't be separated. The study analyzed seed-borne fungi of imported grass seeds from 5 countries, and the ITS sequences were applied for seed-borne fungi of imported grass seeds preliminary detection and identification."
Language:Chinese
References:25
Note:Abstract also appears in English
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lei, Y.-h., W.-g. Kuang, C.-s. Zheng, W.-c. Wang, C.-j. Li, and W.-n. Gao. 2016. Detection and identification of seed-borne fungi isolated from imported grass seeds. (In Chinese) [Caoye Kexue] [Pratacultural Science]. 33(1):p. 46-53.
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http://cykx.lzu.edu.cn//EN/article/downloadArticleFile.do?attachType=PDF&id=11322
    Last checked: 05/20/2016
    Requires: PDF Reader
http://cykx.lzu.edu.cn//article/2016/1001-0629-33-1-46.html
    Last checked: 05/20/2016
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