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| DOI: | 10.1094/PHYTO.2011.101.6.S1 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2011.101.6.S1#page=54
Last checked: 06/17/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Florea, S.;
Mulinti, P.;
Panaccione, D. G.;
Schardl, C. L. |
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| Author Affiliation: | Florea and Schardl: Department of Plant Pathology, University of Kentucky, Lexington, KY; Mulinti and Panaccione: Division of Plant & Soil Sciences, West Virginia University, Morgantown, WV |
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| Title: | Ergot alkaloid gene expression studies in a grass-endophyte association |
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| Section: | 2011 APS-IPPC Joint Meeting abstracts of presentations
Other records with the "2011 APS-IPPC Joint Meeting abstracts of presentations" Section
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| Meeting Info.: | Honolulu, Hawaii: August 6-10, 2011 |
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| Source: | Phytopathology. Vol. 101, No. 6s, June 2011, p. S54. |
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| Publishing Information: | Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Alkaloid accumulation; Endophytes; Epichloe; Ergot alkaloids; Gene expression; Neotyphodium; Tissue testing
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| Abstract/Contents: | "Many epichloid endophytes (Epichloe and Neotyphodium spp.) are systemic symbionts of cool-season grasses, providing protection against vertebrate and invertebrate herbivores by producing mycotoxins. Among these compounds are the ergot alkaloids (EA) responsible for livestock toxicosis. The outcome of the EA pathway is a complex profile of alkaloids consisting of the pathway end product and several intermediates that can accumulate at levels comparable the end products. These endophytes grow vegetatively throughout aerial tissues of infected plants without morphological differentiation. However, there is a possibility that fungal hyphae in different tissues have different gene expression patterns. To test this hypothesis, we conducted a reverse-transcription-quantitative PCR study of the N. lolii ^D#x E. typhina hybrid isolate Lp1 in perennial ryegrass to measure the expression of six EA biosynthesis genes (dmaW, easC, easD, easA, cloA, and lpsA) in grass pseudostems, center leaves, outer leaves, leaf blades, and developing seeds. For each gene, tissue samples from at least three individual plants were analyzed. Possible relationships between gene expression levels and ergot alkaloid profiles were examined. Within the same plant tissue all examined genes revealed similar expression profiles, whereas expression levels differed widely among different tissues. There was no definite correlation between the differences in gene expression and the profile of alkaloids accumulating in different tissues." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Florea, S., P. Mulinti, D. G. Panaccione, and C. L. Schardl. 2011. Ergot alkaloid gene expression studies in a grass-endophyte association. Phytopathology. 101(6s):p. S54. |
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| DOI: 10.1094/PHYTO.2011.101.6.S1 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2011.101.6.S1#page=54
Last checked: 06/17/2016
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b2219736a |
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Digitally in TIC by file name: phytp2011junpres |
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