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| DOI: | 10.1094/PHYTO.2011.101.6.S264 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2011.101.6.S264#page=4
Last checked: 06/20/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Ma, B.;
Tredway, L. P. |
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| Author Affiliation: | North Carolina State University |
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| Title: | The duration of DMI exposure affects the expression of ShCYP51 differently in sensitive and insensitive isolates of Sclerotinia homoeocarpa |
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| Section: | 2011 Southern Division meeting abstracts
Other records with the "2011 Southern Division meeting abstracts" Section
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| Meeting Info.: | Corpus Christi, Texas: February 6-7, 2011 |
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| Source: | Phytopathology. Vol. 101, No. 6S, June 2011, p. S267. |
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| Publishing Information: | Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: DMI fungicides; DMI resistance; Gene expression; Gene isolation; Sclerotinia homoeocarpa
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| Abstract/Contents: | "DMI insensitivity or resistance has become widespread in populations of Sclerotinia homoeocarpa, the causal agent of dollar spot in turfgrasses. The induced expression of ShCYP51 as influenced by the length of DMI exposure was quantified in six sensitive and six insensitive isolates. ShCYP51 expression increased in all isolates after exposure to propiconazole at 0.5 μg/ml for 1 h, 24 h or 48 h when compared to the constituitive level of expression. However, 24 h and 48 h exposures resulted in significantly higher levels of expression in the insensitive group than in the sensitive group. In addition, expression of the gene was greater at 48 h than at 24 h in the insensitive group, whereas no corresponding increase was observed in the sensitive group. Comparing expression of ShCYP51 among individual isolates, no differences were detected in constituitive expression or after 1 h propiconazole exposure. Expression was greatly increased for all isolates after 24 h of exposure, but after 48 h of propiconazole exposure, five of six insensitive isolates exhibited higher levels of expression than six sensitive isolates. Results from this study show that over-expression of ShCYP51 following DMI exposure is an important factor determining DMI sensitivity in S. homoeocarpa. In addition, the findings that the level of ShCYP51 expression was positively correlated with the duration of DMI exposure suggests that increased rate or frequency of DMI fungicide application could magnify ShCYP51 expression levels and further reduce disease control." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ma, B., and L. P. Tredway. 2011. The duration of DMI exposure affects the expression of ShCYP51 differently in sensitive and insensitive isolates of Sclerotinia homoeocarpa. Phytopathology. 101(6S):p. S267. |
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| DOI: 10.1094/PHYTO.2011.101.6.S264 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2011.101.6.S264#page=4
Last checked: 06/20/2016
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b2219736a |
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Digitally in TIC by file name: phytp2011junsoutherndiv |
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