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| DOI: | 10.1094/PHYTO.2011.101.6.S1 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2011.101.6.S1#page=147
Last checked: 06/20/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Putman, A. I.;
Carbone, I.;
Tredway, L. P. |
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| Author Affiliation: | North Carolina State University, Raleigh, NC |
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| Title: | Development and characterization of microsatellite markers for Sclerotinia homoeocarpa |
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| Section: | 2011 APS-IPPC Joint Meeting abstracts of presentations
Other records with the "2011 APS-IPPC Joint Meeting abstracts of presentations" Section
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| Meeting Info.: | Honolulu, Hawaii: August 6-10, 2011 |
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| Source: | Phytopathology. Vol. 101, No. 6S, June 2011, p. S147. |
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| Publishing Information: | Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Disease profile; Dollar spot; Genetic diversity; Genetic markers; Microsatellites; Sclerotinia homoeocarpa
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| Abstract/Contents: | "Sclerotinia homoeocarpa causes dollar spot, the most economically important disease of turfgrass worldwide. The objective of this research is to develop and use microsatellite markers in population studies of S. homoeocarpa. Microsatellites were initially isolated using a bead capture enrichment protocol, and additional repeats were identified in silico from an early draft genome assembly of S. homoeocarpa using the Tandem Repeat Database. Microsatellites with sufficient flanking sequence to the end of the read or to adjacent repeats were deemed suitable for primer design. Next, candidate loci were examined for polymorphisms by Sanger sequencing. Candidates containing indels in the flanking region or compound polymorphisms were discarded as not usable. From the genome data, 6,075 microsatellites were identified based on minimum thresholds of repeat number, copy number, and perfection of repeat units. Two of 31 candidate loci from the enrichment protocol were selected as usable. Of the 791 candidate loci identified in silico, to date 5 usable loci have been selected and 42 have been discarded. Two to four alleles per locus have been found among a select group of cool- and warm-season isolates from four continents. Multiplex PCR protocols using fluorescently-tagged universal primers are being developed to enable rapid genotyping. These microsatellites will be useful in determining the diversity and structure among worldwide populations of S. homoeocarpa. |
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| Language: | English |
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| References: | 0 |
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| See Also: | Updated reprint appears in 2011 International Annual Meetings: [Abstracts][ASA-CSSA-SSSA]. 2011, p. 66854, R=192813. R=192813 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Putman, A. I., I. Carbone, and L. P. Tredway. 2011. Development and characterization of microsatellite markers for Sclerotinia homoeocarpa. Phytopathology. 101(6S):p. S147. |
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| DOI: 10.1094/PHYTO.2011.101.6.S1 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2011.101.6.S1#page=147
Last checked: 06/20/2016
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b2219736a |
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