Full TGIF Record # 273337
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DOI:10.1016/j.scienta.2016.05.030
Web URL(s):http://www.sciencedirect.com/science/article/pii/S0304423816302734
    Last checked: 07/13/2016
    Access conditions: Item is within a limited access website
Publication Type:
i
Refereed
Author(s):Zhang, Bing; Shi, Jin-Ang; Chen, Jing-Bo; Li, Dan-Dan; Li, Jian-Jian; Guo, Hai-Lin; Zong, Jun-Qin; Wang, Yi; Guo, Ai-Gui; Liu, Jian-Xiu
Author Affiliation:Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China
Title:Efficient virus-induced gene silencing in Cynodon dactylon and Zoysia japonica using rice tungro bacilliform virus vectors
Source:Scientia Horticulturae. Vol. 207, August 5 2016, p. 97-103.
# of Pages:7
Publishing Information:Amsterdam, Elsevier
Keywords:TIC Keywords: Controlling genes; Cynodon dactylon; Gene expression; Gene repression; Genetic transformation; Viruses; Zoysia japonica
Abstract/Contents:"Cynodon dactylon and Zoysia japonica are two important warm-season turfgrass species with multiple utilizations. Genetic transformations of Cynodon dactylon and Zoysia japonica are extremely difficult, which severely restrict the gene functional studies and molecular breeding research of the two grasses. By contrast, virus-induced gene silencing (VIGS) presents an effective and rapid alternative method to create targeted gene knock-outs for gene functional studies in plants. In this study, we tested the efficiency of modified rice tungro bacilliform virus (RTBV) vector in silencing Cynodon dactylon and Zoysia japonica phytoene desaturase (PDS) gene through agroinfiltration. The agroinfiltrated leaves of two Cynodon dactylon and two Zoysia japonica cultivars all showed white streaks typical for PDS gene silencing at 21 days post agroinfiltration. In Cynodon dactylon and Zoysia japonica plants displaying white streak leaf symptoms, PDS gene expression in leaves were all significantly lower than that of control and mock-infected plants. These results indicated that RTBV-VIGS system is efficient in Cynodon dactylon and Zoysia japonica."
Language:English
References:24
Note:Pictures, color
Figures
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhang, B., J.-A. Shi, J.-B. Chen, D.-D. Li, J.-J. Li, H.-L. Guo, et al. 2016. Efficient virus-induced gene silencing in Cynodon dactylon and Zoysia japonica using rice tungro bacilliform virus vectors. Scientia Horticulturae. 207:p. 97-103.
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DOI: 10.1016/j.scienta.2016.05.030
Web URL(s):
http://www.sciencedirect.com/science/article/pii/S0304423816302734
    Last checked: 07/13/2016
    Access conditions: Item is within a limited access website
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MSU catalog number: b4895016~S1a
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