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Web URL(s):http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=22
    Last checked: 07/20/2016
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Author(s):Jespersen, David; Bonos, Stacy A.; Belanger, Faith C.; Raymer, Paul; Huang, Bingru
Author Affiliation:Jespersen and Raymer: Department of Crop and Soil Sciences, University of Gerogia; Bonos, Belanger, Huang: Department of Plant Biology and Pathology, Rutgers University
Title:Characterization and validation of molecular markers linked to heat and drought tolerance for marker assisted selection of stress-tolerant creeping bentgrass
Section:Plenary presentations
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Meeting Info.:New Brunswick, New Jersey: March 18, 2016
Source:Proceedings of the Twenty-Fifth Annual Rutgers Turfgrass Symposium. Vol. 25, 2016, p. 22.
Publishing Information:New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey
# of Pages:1
Keywords:TIC Keywords: Abiotic injury; Agrostis stolonifera; Breeding aims; Cultivar improvement; Drought resistance; Genetic markers; Heat resistance; Microsatellites; Molecular markers; Normalized Difference Vegetation Index; Physiological responses; Quantitative trait loci; Simple sequence repeats; Stress tolerance
Abstract/Contents:"Creeping bentgrass (Agrostis stolonifera L.) is a cool-season turfgrass valued for its dense, fine canopy and ability to withstand low mowing heights. Due to these desirable characteristics creeping bentgrass is frequently used for high value turf areas such as golf course greens and fairways. Unfortunately creeping bentgrass has low to moderate stress tolerance which can limit its performance and use during periods of abiotic stress. Two major abiotic stresses affecting creeping bentgrasses are drought and heat which lead to reductions in growth, premature leaf senescence and eventual plant death. The development of elite cultivars with improved stress tolerance would allow plants to maintain greater turf quality during stress periods with fewer inputs, as well as potentially allowing turf managers in southern altitudes to take advantage of creeping bentgrass's superior turf characteristics. One way to develop improved cultivars is through the use of marker assisted selection (MAS) which utilizes molecular markers linked to important traits resulting in improved selection speed and efficiency. Potential molecular markers have been identified using quantitative trait loci (QTL) analysis on existing creeping bentgrass linkage maps developed at Rutgers University. Additionally several candidate gene markers have also been developed which correspond to genes previously implicated to play important roles in stress tolerance, such as stress defense genes coding for heat shock proteins, or genes involved with photosynthesis and energy production. These markers include microsatellite or simple sequence repeat (SSR) markers as well as gene specific markers using cleaved amplified polymorphic sequences (CAPS) and Ecotilling methodologies. Using these previously identified markers, a diverse population of creeping bentgrass germplasm consisting of 127 individuals was screened during 2014 and 2015 at both the University of Georgia and Rutgers University for important physiological traits associated with drought and heat tolerance. These traits include leaf membrane stability, chlorophyll content, green leaf biomass as estimated by normalized difference vegetation index (NDVI), as well as visual ratings of turf quality. IN addition to identifying individuals with improved levels of stress tolerance, association analysis was performed to determine if the previously identified molecular markers were associated with important physiological traits for drought or heat tolerance in this new population. Markers significantly associated with important physiological traits for stress tolerance may be linked with important mechanisms for abiotic stress tolerance and be used in MAS to develop improved cultivars. The confirmations of these markers will not only support their utility for use in MAS protocols, but may also be useful for identifying key mechanisms for abiotic stress tolerance."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Jespersen, D., S. A. Bonos, F. C. Belanger, P. Raymer, and B. Huang. 2016. Characterization and validation of molecular markers linked to heat and drought tolerance for marker assisted selection of stress-tolerant creeping bentgrass. Proc. Rutgers Turfgrass Symp. 25:p. 22.
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http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=22
    Last checked: 07/20/2016
    Requires: PDF Reader
    Notes: Item is within a single large file
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