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Web URL(s):http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=46
    Last checked: 07/20/2016
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Author(s):Averello, Vincenzo; Kubik, Christine; Vaiciunas, Jennifer; Bonos, Stacy A.; Meyer, William A.; Honig, Josh A.
Author Affiliation:Department of Plant Biology and Pathology, Rutgers University
Title:Classification of tall fescue (Festuca arundinacea Schreb.) using nuclear microsatellite (nuSSR) markers
Section:Poster presentations
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Meeting Info.:New Brunswick, New Jersey: March 18, 2016
Source:Proceedings of the Twenty-Fifth Annual Rutgers Turfgrass Symposium. Vol. 25, 2016, p. 46.
Publishing Information:New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey
# of Pages:1
Keywords:TIC Keywords: Festuca arundinacea; Genetic markers; Genomics; Germplasm; Microsatellites; Polymerase chain reaction; Simple sequence repeats
Abstract/Contents:"Tall fescue (Festuca arundinacea Schreb.) is an allohexaploid (2n=6x=42) grass (family Poaceae) that has applications for turf and forage. The native range of hexaploid tall fescue is from northern Europe though North Africa. It occurs in three morphotypes: 1) the continental morphotype, which is most predominant and the type from which most turfgrass cultivars were developed, occurring in northern Europe through Turkey; 2) the Mediterranean morphotype and the other morphotypes. The genomic constitution of the continental type is G1G1G2G2PP, with the G1 and G2 genomes coming from tetraploid tall fescue (Festuca arudinacea var. glaucescens Boiss) and the P genome coming from meadow fescue (Festuca pratensis (Huds.) P. Beauv.). Since the 1930s and the release of 'Kentucky 31', tall fescue has been widely used as a forage; and since 1981 with the release of 'Rebel', tall fescue has been extensively bred for turf use. Breeding efforts have enhanced its drought and heat tolerance, as well as produced cultivars with fine leaf texture allowing this species to become popular for home lawns and sports fields. The objective of this study was to determine the genetic relatedness between cultivars and collections of tall fescue using nuclear SSR (nuSSR) markers. Samples of 104 tall fescue cultivars and 66 collected from turf plots at Adelphia, NJ< and separated into individual tillers. Tillers were maintained in a greenhouse for later DNA extraction. Perennial ryegrass (Lolium perenne L.), annual ryegrass (Lolium multiflorum Lam.), tetraploid tall fescue, and meadow fescue were used as outgroups for phylogenetic analysis. These outgroups were grown from seeds distributed from USDA ARS-GRIN. Complete genomic DNA of all samples was extracted with either the Sigma GenElute Plant Genomic DNA Miniprep Kit or the Quiagen DNeasy 96 Plant Kit according to the manufacturer's instructions. 29 nuSSR markers generated a total of 414 polymorphic bands in the samples of the current study. PCR product size from each individual was assessed using capillary electrophoresis (Applied Biosystems 3500 XL Genetic Analyzer). Analysis of Molecular Variance (AMOVA) results indicated that 75% of the genetic diversity was found within populations and 25% was partitioned among populations. A neighbor-joining (NJ) tree was generated using the pairwise Φpt results from the AMOVA analysis. NJ results showed that group by location of origin, and were genetically distinct from each other. 'Rhambler' and 'Sixpoint' were the only two cultivars not distinguishable by the current set of nuSSR markers. Collections showed greater within population diversity than the cultivars, indicating that these collections could be a good source of new and diverse germplasm. Results indicate that this set of SSR markers can be used in the future to fingerprint new cultivars and collections as they are generated or acquired."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Averello, V., C. Kubik, J. Vaiciunas, S. A. Bonos, W. A. Meyer, and J. A. Honig. 2016. Classification of tall fescue (Festuca arundinacea Schreb.) using nuclear microsatellite (nuSSR) markers. Proc. Rutgers Turfgrass Symp. 25:p. 46.
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Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=46
    Last checked: 07/20/2016
    Requires: PDF Reader
    Notes: Item is within a single large file
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