Full TGIF Record # 273461
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Web URL(s):http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=47
    Last checked: 07/21/2016
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Author(s):Bonos, Stacy A.; Koch, Eric; Vaiciunas, Jennifer; Honig, Josh A.; Meyer, William A.; Zelzion, Udi; Bhattacharya, Debashish
Author Affiliation:Bonos, Vaiciunas, Honig and Meyer: Department of Plant Biology and Pathology; Bhattacharya: Department of Ecology and Natural Resources, Rutgers University; Koch: The Scotts Company, Marysville, Ohio
Title:Differential gene expression of salt-stressed perennial ryegrass
Section:Poster presentations
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Meeting Info.:New Brunswick, New Jersey: March 18, 2016
Source:Proceedings of the Twenty-Fifth Annual Rutgers Turfgrass Symposium. Vol. 25, 2016, p. 47-48.
Publishing Information:New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey
# of Pages:2
Keywords:TIC Keywords: Epichloe festucae; Gene mapping; Genomics; Genotypes; Heat shock proteins; Lolium perenne; Salt tolerance; Stress response
Abstract/Contents:"The development of turfgrass cultivars that can tolerate salinity while maintaining safe, acceptable quality would result in a community and industry more accepting of voluntary utilization of alternative water sources. Salinity tolerance could involve a number of processes including the regulation or expression of compatible solutes/osmolytes, polyamines, reactive oxygen species, antioxidant defense mechanisms, ion transport and compartmentalization of injurious ions (Mudgal et al., 2010). Unfortunately, current breeding efforts to develop salt tolerant turfgrasses have been slow and the mechanism for salinity tolerance is not fully understood. The goal of this project was to study gene expression of salinity tolerant and susceptible perennial ryegrass (Lolium perenne L.) clones by sequencing the transcriptome of salt-stressed and non-stressed leaves. Two genotypes, S1 - salt tolerant and BS5 - salt sensitive, were selected based on a previous salinity tolerance screening. These genotypes were vegetatively propagated into three replicates and grown under control and salinity stress conditions in a greenhouse using greenhouse salinity screening technique (Koch and Bonos, 2011). At the onset of salt stress symptoms, leaf tissue samples were cut and immediately flash frozen in liquid nitrogen. RNA was extracted using RNeasy Plant Mini Kit and sequencing libraries were constructed using TruSeq RNA Sample Preparation Kit v2 by Illumina Inc. according to the manufacturer's instructions. Library samples were then run on a MiSeq (Illumina Inc.) benchtop sequencer. The raw reads from all samples were quality-trimmed to retain only high quality reads; To filter out possible fungal (Epichloe festucae Leuchtm., Shardl & M. R. Siegel) and Lolium perenne organelle (Chloroplast and Mitochondrion) reads, we aligned the trimmed reads to the Epichloe festucae genome sequence and to the Lolium perenne organelle sequences. The two genotype (S1 and BS5) transcriptome (50,073 contigs) and the number of reads mapped per contig were counted and used as input to DESeq2, an R/Bioconductor package (Anders and Huber, 2010) which infers differential expression (DE) based on the negative binomial distribution. For this analysis we used a cutoff of 5% to control for false detection rate (FDR, false positives), and considered only genes that had a log2-fold change ā‰„Ā±2 and FDR <0.05 to be differentially expressed. Two thousand two hundred and ninety-six genes were differently expressed (DE) as a result of salt stress in the S1 genotype (tolerant), while the same comparison in the BS5 genotype resulted in 940 DE genes. The majority of the genes that were strongly upregulated in the salt tolerant genotype S1, were in the dehydrin (or LEA [Late Embryogenesis Abundant]) family of genes including drought acclimating dehydrin WZY2, Dehydrin DHN3, and LEA proteins. LEA proteins are believed to participate in protecting cellular components from dehydration in response to drought, salt or cold tolerance (Vaseva et al. 2011). The dehydrins and LEA proteins were strongly upregulated only in the salt tolerant genotype indicating that the tolerant genotype, through the production of these proteins, may be able to reduce dehydration induced damage or chelate ions, to alleviate the damaging effect of increased ion concentrations (Danyluk et al., 1998). These genes have been found to be produced earlier in tolerant genotypes than susceptible genotypes (Vaseva et al., 2011) (which is what we identified in the research). This suggests that salt tolerant perennial ryegrasses may have a better ability to prevent damage to proteins at the early onset of stress. In the salt susceptible genotype BS5, several heat shock proteins, beta glucosidase, and cinnamoyl CoA reductase among others were preferentially upregulated. These genes have been associated with stress response in other crops. There were no LEA or dehydrin proteins produced in the susceptible genotype indicating that these genes could potentially be used as markers to screen germplasm for salinity tolerance in perennial ryegrass. We are hopeful that this project will bring us closer to identifying mechanisms involved with salinity tolerance and potentially identify sequence variation that can be used in the future for genomic selection in perennial ryegrass."
Language:English
References:6
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bonos, S. A., E. Koch, J. Vaiciunas, J. A. Honig, W. A. Meyer, U. Zelzion, et al. 2016. Differential gene expression of salt-stressed perennial ryegrass. Proc. Rutgers Turfgrass Symp. 25:p. 47-48.
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http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=47
    Last checked: 07/21/2016
    Requires: PDF Reader
    Notes: Item is within a single large file
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