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| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=52
Last checked: 07/21/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Di, Rong;
Bonos, Stacy |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Gene editing of creeping bentgrass to improve stress tolerance and disease resistance |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, New Jersey: March 18, 2016 |
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| Source: | Proceedings of the Twenty-Fifth Annual Rutgers Turfgrass Symposium. Vol. 25, 2016, p. 52. |
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| Publishing Information: | New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Cultivar improvement; Disease resistance; Dollar spot; Genetic engineering; Sclerotinia homoeocarpa; Stress tolerance
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| Cultivar Names: | Crenshaw |
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| Abstract/Contents: | "Creeping bentgrass (Agrostis stolonifera L.) is one of the most widely used cool-season grass species on golf courses. However, there is not one cultivar of creeping bentgrass that is considered completely resistant to dollar spot disease caused by Sclerotinia homoeocarpa F. T. Bennet. Creeping bentgrass is also susceptible to heat and drought stress during summer months. In this project, we used the new CRISPR/Cas (clustered regularly interspaced short palindromic repeats) -associated endonuclease gene editing technology to knock-out disease susceptibility and stress-related genes in creeping bentgrass to improve disease resistance and stress tolerance. Using bioinformatics tools, we have identified EST (expressed sequence tag) sequences for the following three genes that are related to disease and stress susceptibility in creeping bentgrass: CPK12-like (CPKL), BONZAI1-like (BONL) and DREB1C-like (DREBL). We have subsequently cloned the partial cDNAs of these three genes from 'Crenshaw' creeping bentgrass. The CRISPR vectors containing the sgRNA (single guide RNA) sequences to target these genes have been constructed. We have also developed the tissue culture system for Crenshaw creeping bentgrass transformation and are in the process of transforming callus tissues with the CRISPR vectors." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Di, R., and S. Bonos. 2016. Gene editing of creeping bentgrass to improve stress tolerance and disease resistance. Proc. Rutgers Turfgrass Symp. 25:p. 52. |
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| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=52
Last checked: 07/21/2016
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b3696858 |
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