Full TGIF Record # 273468
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Web URL(s):http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=53
    Last checked: 07/21/2016
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Report
Author(s):Gianfagna, Thomas J.; Meyer, William A.; Peters, Jeanne S.; White, James F.; Mohr, Melissa
Author Affiliation:Plant Biology and Pathology Department, Rutgers University
Title:Chemical and biological analysis of new tall fescue germplasm for turf and pasture use
Section:Poster presentations
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Meeting Info.:New Brunswick, New Jersey: March 18, 2016
Source:Proceedings of the Twenty-Fifth Annual Rutgers Turfgrass Symposium. Vol. 25, 2016, p. 53.
Publishing Information:New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey
# of Pages:1
Keywords:TIC Keywords: Claviceps purpurea; Endophyte-infected plants; Epichloe; Festuca arundinacea; Microscopy
Abstract/Contents:"The focus of our work in 2015 was to compare and evaluate microscopy with immunochemistry for the identification of endophytes. This was prompted by concerns over the accuracy of the widely used Agrinostics Immunochemical test kit for endophyte detection. Tall fescue (Festuca arundinacea Screb.) selections can contain the endophyte Epichloμe coenophiala (Morgan-Jones & W. Gams) C. W. Bacon & Schardl, 2015. We screened 1440 individual tillers from 30 selections of tall fescue, each with 24 plants/selection, for endophyte. Many of the selections (19) contained a mix of endophyte positive and negative plants. We conducted a blind test using plants from the mixed groups for the presence of the endophyte by microscopy. In each and every case, the results from microscopy were identical to the immunochemical test kit results. Nevertheless, both methods have limitations. The test kit is known to cross react with the closely related pathogen Claviceps purpurea (Fries) Tulasne and perhaps other species in the Claviceptaceae, whereas microscopy can fail to identify low titer samples containing endophytes, and it may be difficult to distinguish the Epichloμe hyphae from other similar fungi present in seeds or tillers. Immunochemical screening is the most efficient method when large numbers of tillers or seeds need to be examined."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Gianfagna, T. J., W. A. Meyer, J. S. Peters, J. F. White, and M. Mohr. 2016. Chemical and biological analysis of new tall fescue germplasm for turf and pasture use. Proc. Rutgers Turfgrass Symp. 25:p. 53.
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Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=53
    Last checked: 07/21/2016
    Requires: PDF Reader
    Notes: Item is within a single large file
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MSU catalog number: b3696858
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