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| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=70
Last checked: 07/26/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Tian, Zipeng;
Wang, Ruying;
Clarke, Bruce B.;
Belanger, Faith C. |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Characterization of an Epichloë festucae Leuchtm., Schardl, M.R. Siegel antifungal protein |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, New Jersey: March 18, 2016 |
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| Source: | Proceedings of the Twenty-Fifth Annual Rutgers Turfgrass Symposium. Vol. 25, 2016, p. 70. |
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| Publishing Information: | New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Disease resistance; Dollar spot; Endophyte-infected plants; Endophytic fungi; Epichloe festucae; Festuca rubra subsp. rubra; Hygromycin; SOLiD-SAGE; Sclerotinia homoeocarpa
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| Abstract/Contents: | "Strong creeping red fescue (Festuca rubra subsp. rubra L.) is an important low maintenance turfgrass species. Strong creeping red fescue plants are often naturally infected with the fungal endophyte Epichloë festucae Leuchtm., Schardl & M.R. Siegel. Endophyte infection confers insect and disease resistance to the host grass. Endophyte-mediated disease resistance is not a general feature of other grass/endophyte interactions and the basis of the disease resistance is currently unknown. An abundantly expressed transcript for a protein similar to Penicillium antifungal protein was recovered from a SOLiD-SAGE transcriptome of E. festucae-infected F. rubra. Genes similar to the E. festucae antifungal protein are not found in most Epichloë spp. for which whole genome sequences are available. The uniqueness of the E. festucae antifungal gene and its transcript abundance makes it a candidate factor involved in the observed disease resistance of endophyte-infected F. rubra. We are currently characterizing the antifungal protein and have partially purified it from apoplastic proteins isolated from endophyte-infected plants. The partially purified protein exhibited antifungal activity against the dollar spot pathogen (Sclerotinia homoeocarpa F.T. Bennett) in plate assays. We have expressed the protein in yeast in order to obtain sufficient quantities of the protein for additional antifungal assays. The antifungal protein expressed in yeast also exhibited antifungal activity against the dollar spot pathogen. We are also attempting to knockout the antifungal protein gene in order to assess its effect on dollar spot disease resistance in endophyte-infected turf. Hygromycin-resistant transformants have been recovered and are currently being screened to identify a knockout transformant for further testing." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tian, Z., R. Wang, B. B. Clarke, and F. C. Belanger. 2016. Characterization of an Epichloë festucae Leuchtm., Schardl, M.R. Siegel antifungal protein. Proc. Rutgers Turfgrass Symp. 25:p. 70. |
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| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2016.pdf#page=70
Last checked: 07/26/2016
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b3696858 |
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Digitally in TIC by file name: rutsy2016 |
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