Full TGIF Record # 274699
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DOI:10.1094/PDIS-01-15-0083-PDN
Web URL(s):http://apsjournals.apsnet.org/doi/full/10.1094/PDIS-01-15-0083-PDN?prevSearch=%24%7BresultBean.text%7D&searchHistoryKey=%24%7BsearchHistoryKey%7D
    Last checked: 10/21/2016
    Notes: Abstract only
Publication Type:
i
Refereed
Author(s):Wang, S. J.
Author Affiliation:College of Agriculture, Hainan University, Haikou, China
Title:First report of take-all root rot caused by Gaeumannomyces graminis var. graminis on Paspalum vaginatum in China
Section:Disease notes: Diseases caused by fungi and fungus-like organisms
Other records with the "Disease notes: Diseases caused by fungi and fungus-like organisms" Section
Source:Plant Disease. Vol. 99, No. 12, December 2015, p. 1858.
Publishing Information:[Washington, D.C.]: Bureau of Plant Industry, U.S. Dept. of Agriculture
# of Pages:1
Keywords:TIC Keywords: Disease evaluation; Disease identification; Gaeumannomyces graminis; Paspalum vaginatum; Pythium diseases
Cultivar Names:Salam
Geographic Terms:China
Abstract/Contents:"Take-all root rot caused by Gaeumannomyces graminis (Sacc.) Arx & Olivier var. graminis has been reported as a destructive disease on several warm-season turfgrass species in temperate regions throughout the world (Smiley 2005). In September 2013, a decline of P. vaginatum was observed on two golf courses in Hainan Province, China. Infected turfgrasses appeared in irregularly shaped, chlorotic patches with individual plants having chlorotic foliage and dark, necrotic lesions on roots and associated stolons. Roots and stolons were often covered with dark, ectotrophic fungi, and lobed hyphopodia. Diseased roots were rotted and often shorter than healthy roots. Samples of P. vaginatum exhibiting decline symptoms were obtained from a golf course fairway in Haikou, Hainan Province. Symptomatic root tissues of P. vaginatum were surface-disinfested in 1% sodium hypochlorite for 1 min, rinsed three times with sterile water, and transferred to potato dextrose agar (PDA) amended with 0.01% gentamicin sulfate. One isolate of Gaeumannomyces sp. (TD-GGG1) with dark hyphae and curled colony edges was selected for further study. Morphological identification including black, flask-shaped perithecia and filiform, septate ascospores of the fungus was consistent with the description of G. graminis var. graminis by Walker (Walker 1980; Walker 2002). The rDNA internal transcribed spacer (ITS) regions of the isolate (TD-GGG1) were amplified using primer pair ITS1/ITS4. The nucleotide sequence of the isolate was assigned the GenBank Accession No. KP404099. A BLAST search showed that the sequence had >99% similarity to G. graminis var. graminis (Accession No.AY428781). To confirm pathogenicity, inocula were prepared by incubating autoclaved sand and corn meal (250 g of sand and 50 g of corn meal) with the isolate for 4 weeks at 25°C. Ten grams of colonized sand and corn meal were incorporated into a peat moss/sand mixture in 10-cm-diameter pots. Eight asymptomatic P. vaginatum (cv. 'Salam') sprigs were washed and planted in infested and noninfested soil as a control, with four replications of each treatment. At 6 weeks after inoculation in a greenhouse, chlorosis appeared on lower leaves and coleoptiles on the plants grown in infested soil. The cortex of roots and stolons were rotted. Both brown runner hyphae and strongly lobed hyphopodia were observed on roots of the diseased plants, while no such symptoms developed on noninoculated controls. Koch's postulates were fulfilled by reisolating the fungus from the symptomatic plants and the culture was confirmed as G. graminis var. graminis by its ITS sequence and the morphological characters. To the best of our knowledge, this is the first report of take-all root rot caused by G. graminis var. graminis on P. vaginatum in China."
Language:English
References:3
See Also:Other items relating to: Seashore Paspalum - Since 2000
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, S. J. 2015. First report of take-all root rot caused by Gaeumannomyces graminis var. graminis on Paspalum vaginatum in China. Plant Disease. 99(12):p. 1858.
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DOI: 10.1094/PDIS-01-15-0083-PDN
Web URL(s):
http://apsjournals.apsnet.org/doi/full/10.1094/PDIS-01-15-0083-PDN?prevSearch=%24%7BresultBean.text%7D&searchHistoryKey=%24%7BsearchHistoryKey%7D
    Last checked: 10/21/2016
    Notes: Abstract only
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