Full TGIF Record # 274904
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Web URL(s):http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=62
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Publication Type:
i
Report
Author(s):Dinkins, Randy; Nagabhyru, Padmaja; Schardl, Christopher
Author Affiliation:Dinkins: Forage-Animal Production Research Unit, USDA-ARS; Nagabhyru and Schardl: University of Kentucky, Lexington, Kentucky
Title:Gene expression and metabolite analysis of endophyte infected and uninfected tall fescue clone pairs under water deficit conditions
Section:Other invited oral presentation abstracts
Other records with the "Other invited oral presentation abstracts" Section
Meeting Info.:Salt Lake City, Utah: June 4-7, 2012
Source:Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. 2012, p. 62.
# of Pages:1
Publishing Information:s.l.: s.n.
Keywords:TIC Keywords: Endophyte-infected plants; Endophytic fungi; Evaluations; Festuca arundinacea; Gene expression; Hosts of plant pests; Water stress
Abstract/Contents:"Tall fescue (Lolium arundinaceum L.) plants symbiotic with the endophytic fungus, Neotyphodiumcoenophialum (E+), have better survivability and persistence under stressful conditions, especially under drought stress, than plants lacking the endophyte (E-). To understand more about the grass-endophyte interactions and how endophyte affects the host plant physiology and gene expression, we conducted a time course water deficit stress experiment using genetically identical E+ and E- clone pairs of tall fescue. Upon re-watering, survival and retillering was significantly greater for E+ than E- plants starting from days 2 - 3 of the stress conditions. RNA-Seq analysis of pseudostem derived RNA comparing E+ and E- of Day 2 stress and control plants was done. CLCbio Genomic Workbench was used to generate contigs using the tall fescue cDNA sequences previously submitted to NCBI as a reference. The contig sequences were analyzed using Blastx and Blastn against the Arabidopsis thaliana, Brachypodium distachyon and rice genomes for comparison and putative annotation assignment. Only a few contigs/genes were differentially expressed between the E+ and E- plants under non-stressed conditions, but a large number were differentially expressed (>2X) between E+ and E- plants under the water deficit conditions. In addition, as expected, a large number of contigs/genes were differentially expressed due to the stress treatment. Metabolomic analyses revealed higher accumulation of the free sugars and proline in E+ plants at early days of onset of stress compared to E- plants. Loline alkaloids and mannitol, fungal metabolites, also increased with water stress. Analysis of specific genes is ongoing and results will be presented."
Language:English
References:0
Note:This item is an abstract only!
"ISBN: 978-1-4675-4762-8"
"MBFT2012"
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Dinkins, R., P. Nagabhyru, and C. Schardl. 2012. Gene expression and metabolite analysis of endophyte infected and uninfected tall fescue clone pairs under water deficit conditions. Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. p. 62.
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http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=62
    Last checked: 09/02/2016
    Requires: PDF Reader
    Notes: Item is within a single large file
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