Full TGIF Record # 274906
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Web URL(s):http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=65
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Publication Type:
Author(s):Milla-Lewis, Susana; Zuleta, Maria Carolina; Van Esbroeck, George; Ottley, Bobby; Kenworthy, Kevin
Author Affiliation:Milla-Lewis, Zuleta, and Van Esbroeck: North Carolina State University, Raleigh, North Carolina; Ottley and Kenworthy: University of Florida, Gainesville, Florida
Title:Molecular and cytological evaluation of genetic diversity in St. Augustinegrass
Section:Other invited oral presentation abstracts
Other records with the "Other invited oral presentation abstracts" Section
Meeting Info.:Salt Lake City, Utah: June 4-7, 2012
Source:Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. 2012, p. 65.
# of Pages:1
Publishing Information:s.l.: s.n.
Keywords:TIC Keywords: Breeding aims; Cultivar improvement; Genetic diversity; Ploidy; Stenotaphrum secundatum
Abstract/Contents:"St. Augustinegrass (Stenotaphrum secundatum (Walt.) Kuntze) is a widely used turfgrass species in warm, tropical and subtropical regions of the world. In the present study, flow cytometry, chromosome counts, and AFLP marker analysis were performed in order to assess genetic diversity at the molecular and cytological levels among public sources of Stenotaphrum germplasm. Cytological investigations identified five different ploidy levels (diploid, triploid, aneuploid, tetraploid, and hexaploid) with chromosome numbers ranging from 2n=2x=18 to 2n=6x=54. Lack of agreement between ow cytometry-inferred ploidy level and chromosome counts was observed for higher ploidy genotypes. Therefore, DNA contents of these accessions are not simple multiples of the content of the diploid genome. This may indicate the existence of different genomes in the genus. Over 1700 loci were scanned with 15 AFLP primer pairs. All plant introductions and cultivars could be uniquely identified and the average pair-wise genetic similarity value was relatively low at 0.63. Results from both principal coordinate and cluster analyses showed well defined separation of accessions by ploidy levels. Moreover, results from the AMOVA indicated that 46% of the total variation could be explained by differences between ploidy levels. A clear positive correlation was observed between ploidy level and number of bands scored, with polyploids showing an increased number of bands. These results indicate that variation in chromosome number is an important source of genetic variation in St. Augustinegrass. Information derived from this study will aid in the strategic utilization of Stenotaphrum germplasm in cultivar development."
Note:This item is an abstract only!
"ISBN: 978-1-4675-4762-8"
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Milla-Lewis, S., M. C. Zuleta, G. Van Esbroeck, B. Ottley, and K. Kenworthy. 2012. Molecular and cytological evaluation of genetic diversity in St. Augustinegrass. Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. p. 65.
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    Last checked: 09/02/2016
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    Notes: Item is within a single large file
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