Full TGIF Record # 274912
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Web URL(s):http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=86
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Author(s):Cogan, Noel O. I.; Shinozuka, Hiroshi; Sawbridge, Timothy I.; Spangenberg, German C.; Forster, John W.
Author Affiliation:Dairy Futures Co-operative Research Centre and Department of Primary Industries, Biosciences Research Division; Sawbridge, Spangenberg, and Forster: La Trobe University, Bundoora, Victoria, Australia
Title:Development of a transcriptome atlas for perennial ryegrass (Lolium perenne L.)
Section:Poster abstracts
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Meeting Info.:Salt Lake City, Utah: June 4-7, 2012
Source:Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. 2012, p. 86.
Publishing Information:s.l.: s.n.
# of Pages:1
Keywords:TIC Keywords: Brachypodium distachyon; Gene isolation; Gene mapping; Lolium perenne; Traits; Transcription factors
Abstract/Contents:"The current generation of DNA sequencing platforms permits significant resources and data to be generated rapidly at relatively low cost. A single genotype of perennial ryegrass has been subjected to transcriptome analysis through deep sequencing of cDNA samples derived from multiple distinct tissue types. A total of 0.6 billion sequencing reads from the Illumina HiSeq2000 platform have been generated from 11 different vegetative samples, including leaf, pseudostem and root samples for both terrestrial and subterranean aspects of gene expression. DNA sequence assembly has been performed on the resulting data from each individual tissue sample, as well as an overall assembly using the SOAP de novo package version 1.05. The assembly generated between c. 23,000 and 29,000 scaffolds from each tissue type, and the combined assembly obtained 57,480 scaffolds, summing to in excess of 47 Mb, with an average length of 820 bp. The resulting contigs have been compared, using a BLAST analysis pipeline, to the gene complement from the related model grass species Brachypodium distachyon L. In excess of 85% of all predicted genes and splice variants of the fully sequenced model grass species have been identified in the assembled ryegrass data set. In addition, analysis of the data has enabled tissue specific patterns of expression to be identified, with over 23,000 genes attributed to a broad expression prole, along with smaller cohorts that display tissue-specific expression. The identification of specific transcription regulation patterns will be valuable for candidate gene identification for agronomically relevant traits."
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"ISBN: 978-1-4675-4762-8"
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Cogan, N. O. I., H. Shinozuka, T. I. Sawbridge, G. C. Spangenberg, and J. W. Forster. 2012. Development of a transcriptome atlas for perennial ryegrass (Lolium perenne L.). Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. p. 86.
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    Last checked: 09/02/2016
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    Notes: Item is within a single large file
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