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| Web URL(s): | http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=92
Last checked: 09/02/2016
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Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Rao, Kovi Mallikarjuna [Kovi, Rao Mallikarjuna];
Rognli, Odd Arne;
Sandve, Simen R.;
Ergon, Ashild;
Studer, Bruno;
Asp, Torben;
Kent, Matthew Peter |
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| Author Affiliation: | Rao [Kovi], Rognli, and Sandve: Department of Plant and Environmental Sciences; Ergon and Kent: Norwegian University of Life Sciences, As, Akershus, Norway; Studer: Aarhus University Asp: Department of Molecular Biology and Genetics, Slagelse, Denmark |
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| Title: | Genetic variation, population structure and linkage disequilibrium in populations of perennial ryegrass selected for freezing tolerance |
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| Section: | Poster abstracts
Other records with the "Poster abstracts" Section
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| Meeting Info.: | Salt Lake City, Utah: June 4-7, 2012 |
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| Source: | Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. 2012, p. 92. |
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| Publishing Information: | s.l.: s.n. |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Alleles; Cold resistance; Genetic diversity; Genotyping; Lolium perenne; Polymorphism
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| Geographic Terms: | Europe |
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| Abstract/Contents: | "Freezing tolerance is a trait of major agronomical importance in northern and central Europe. The plant material studied was derived from an experimental population established from pair-crosses and polycrosses among five genotypes of the Lolium Test Set (genotypes LTS 3, 4, 11, 15, and 16), employed in the EU project GRASP to represent diverse trait performance and climatic adaptation in Europe. Three-hundred individual plants from Syn- 0 generation were first screened for freezing tolerance. Then two generations of differential selection (selection intensity 10%) using artificial freezing tests followed by open pollination of the 30 selected genotypes in isolation was conducted generating a high-freezing tolerant (HFT-2) and a low-freezing tolerant (LFT-2) population. Every round of selection was performed using 300 plants from each divergent population. An unselected Syn-2 generation (US) was generated by intercrossed 100 randomly selected individuals every generation to quantify the effect of random genetic drift. A total of 80 individuals (HFT-2: 24, LFT-2: 29 and US: 27) were genotyped using 278 genic Lolium perenne SNPs distributed across the linkage groups as follows: LG1 (37); LG2 (57); LG3 (32); LG4 (40); LG5 (39); LG6 (37); and LG7 (36). The SNP genotyping was performed using the Sequenom MassARRAY iPLEX Gold technology. Linkage disequilibrium (LD) was measured within each chromosome among the three frost tolerant populations and observed higher LD in the selected populations than in the unselected. LD (r2) decay varied among the three populations especially for LG5 and LG7, linkage groups that contain several QTLs/genes for freezing tolerance. Structure analysis further identified subpopulations within the selections. Data for allele frequencies, genetic diversity, and polymorphic information content will be presented and discussed." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only!
"ISBN: 978-1-4675-4762-8"
"MBFT2012" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Rao, K. M. [Kovi, M. R.], O. A. Rognli, S. R. Sandve, A. Ergon, B. Studer, T. Asp, et al. 2012. Genetic variation, population structure and linkage disequilibrium in populations of perennial ryegrass selected for freezing tolerance. Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. p. 92. |
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| Web URL(s):
http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=92
Last checked: 09/02/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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