Full TGIF Record # 274917
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Web URL(s):http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=95
    Last checked: 09/02/2016
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Publication Type:
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Report
Author(s):Islam, Md. Shofiqul; M√łller, Ian Max; Studer, Bruno; Byrne, Stephen; Panitz, Frank; Bendixen, Christian; Asp, Torben
Author Affiliation:Islam, M√łller, and Byrne: Department of Molecular Biology and Genetics, Aarhus University; Studer and Asp: Institute of Molecular Biology and Genetics, Slagelse; Panitz and Bendixen: Department of Molecular Biology and Genetics, Aarhus University, Tjele, Denmark
Title:Sequencing and annotation of the perennial ryegrass mitochondrial genome
Section:Poster abstracts
Other records with the "Poster abstracts" Section
Meeting Info.:Salt Lake City, Utah: June 4-7, 2012
Source:Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. 2012, p. 95.
# of Pages:1
Publishing Information:s.l.: s.n.
Keywords:TIC Keywords: Hybridization; Lolium perenne; Mitochondrial genetics; Reproductive sterility; Turfgrass profile
Abstract/Contents:"Perennial ryegrass (Lolium perenne L.) is one of the most important forage and turf grass species adapted to temperate regions worldwide. As an allogamous species, perennial ryegrass is currently being improved as populations, thereby not fully exploiting the potential of heterosis. The mitochondrial genome of perennial ryegrass was sequenced as part of a project with the long term goal to control pollination for the development of hybrid breeding schemes using cytoplasmic male sterility. Intact mitochondria were isolated from leaves of a four-month-old plant and used for DNA extraction. Mitochondrial DNA was sequenced using the 454 GS-FLX sequencer, resulting in 287,367 single reads with an average read length of 406bp. The reads were quality filtered and contaminating chloroplast sequences were removed from the dataset. De novo assembly using the CLC Genomics Workbench resulted in approximately 2,400 contigs, of which nine contigs ranging from 6,735 to 219,170 bp were identified as mitochondrial sequences. The scaffold information from a draft assembly of the perennial ryegrass genome, which became available in our laboratory, was used to order and orientate the contigs. Primers were designed to PCR amplify across gaps. The resulting fragments were sequenced and used to close gaps between established contigs. Finally, the mitochondrial genome was assembled into a master circle molecule of 678,559 bp. We are currently working on the annotation of the mitochondrial genome and have already identified large duplications in the entire genome, as well as major rearrangements compared to mitochondrial genomes of other grass species. The genetic structure of the mitochondrial genome of perennial ryegrass will be presented. Our work constitutes a solid basis for comparative analyses of mitochondrial genomes within Lolium and between closely related grass species, and will - on the long run - increase our understanding of cytoplasmic male sterility in perennial ryegrass."
Language:English
References:0
Note:This item is an abstract only!
"ISBN: 978-1-4675-4762-8"
"MBFT2012"
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Islam, Md. S., I. M. M√łller, B. Studer, S. Byrne, F. Panitz, C. Bendixen, et al. 2012. Sequencing and annotation of the perennial ryegrass mitochondrial genome. Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. p. 95.
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http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=95
    Last checked: 09/02/2016
    Requires: PDF Reader
    Notes: Item is within a single large file
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