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| Web URL(s): | http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=97
Last checked: 09/02/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Tan, Chengcheng;
Wu, Yanqi;
Taliaferro, Charles;
Bell, Greg;
Martin, Dennis;
Samuels, Timmy |
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| Author Affiliation: | Wu: Department of Plant and Soil Sciences; Tan, Taliaferro, Bell, Martin, and Samuels: Oklahoma State University, Stillwater, Oklahoma |
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| Title: | Development and characterization of genomic simple sequence repeat markers in Cynodon transvaalensis |
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| Section: | Poster abstracts
Other records with the "Poster abstracts" Section
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| Meeting Info.: | Salt Lake City, Utah: June 4-7, 2012 |
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| Source: | Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. 2012, p. 97. |
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| Publishing Information: | s.l.: s.n. |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Cynodon dactylon x Cynodon transvaalensis; Cynodon transvaalensis; Genetic markers; Genetics model; Hybridization; Inheritance; Simple sequence repeats
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| Cultivar Names: | TN 4200 24-2 |
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| Abstract/Contents: | "Cynodon transvaalensis, commonly called 'African bermudagrass', has economic value as a turf grass, and significant breeding value to hybridize with C. dactylon var. dactylon to develop new interspecific clonal turf cultivars. Simple sequence repeat (SSR) markers are one of the most widely used co-dominant molecular marker systems, which should be highly suitable for genetic investigations in highly heterozygous African bermudagrass. However, few SSR markers are available in C. transvaalensis. Accordingly, the objective of this study was to develop genomic SSR markers in the species. Genomic DNA of 'TN 4200 24-2' C. transvaalensis accession was used to construct four SSR genomic libraries enriched with CA, GA, AAG, AAT as core sequences. The SSR-enriched DNA inserts sequenced at Oklahoma State University Core Facility generated 3050 trimmed sequences, which were examined with CAP3 program to eliminate the redundancy and blast against the previously developed SSR markers. Consequently, 1426 non-redundant primer pairs were designed from the sequences with SSR locator software. After the initial screening on 'TN 4200 24-2' genotype, 981 primer pairs were effective in amplifying reproducible clear target bands. A panel of another seven transvaalensis accessions plus the DNA donor genotype was utilized to test polymorphisms of the 981 SSR markers. An inheritance study was conducted in six transvaalensis progeny plants with two parent plants 'Uganda' and 'T577' accessions. The laboratory work of testing SSR markers polymorphism and inheritance was just finished, and more results will be updated." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only!
"ISBN: 978-1-4675-4762-8"
"MBFT2012" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tan, C., Y. Wu, C. Taliaferro, G. Bell, D. Martin, and T. Samuels. 2012. Development and characterization of genomic simple sequence repeat markers in Cynodon transvaalensis. Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. p. 97. |
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| Web URL(s):
http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=97
Last checked: 09/02/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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