Full TGIF Record # 274925
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Web URL(s):http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=114
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Publication Type:
Author(s):Young, Carolyn; Takach, Johanna
Author Affiliation:Young: Forage Improvement Division; Takach: The Samuel Roberts Noble Foundation, Ardmore, Oklahoma
Title:Understanding alkaloid diversity in tall fescue endophtyes [endophytes]
Section:Poster abstracts
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Meeting Info.:Salt Lake City, Utah: June 4-7, 2012
Source:Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. 2012, p. 114.
# of Pages:1
Publishing Information:s.l.: s.n.
Keywords:TIC Keywords: Alkaloid accumulation; Cultivar evaluation; Cultivar variation; Endophytes; Epichloe; Festuca arundinacea; Gene mapping; Stress factors
Abstract/Contents:"Epichloid endophytes (Epichloa^D"nd Neotyphodium sp.) associate with cool-season grasses, including the agriculturally important forages tall fescue and perennial ryegrass. This association confers protection from a variety of biotic and abiotic stresses, including herbivory and drought. A collection of 85 tall fescue lines from 15 locations in Greece, including both Continental (summer active) and Mediterranean (summer dormant) germplasm, was screened for the presence of native endophytes. A total of 37 endophyte-infected lines from 10 locations were identified and the endophytes were classified into five distinct groups based on physical characteristics and molecular markers designed to alkaloid biosynthesis genes and SSR sequences. These classifications were supported by phylogenetic analyses of the housekeeping genes tefA and tubB, and the endophytes were further categorized as N. coenophialum (represented by three independent groups) or Neotyphodium sp. FaTG-2 (represented by two independent groups) isolates. Analyses of the tall fescue matK chloroplast genes indicated a population-wide, host-specific association between N. coenophialum and Continental tall fescue and FaTG-2 with Mediterranean tall fescue, which also correlated with differences in colonization of host tillers by the native endophytes. The alkaloid potential of the endophytes was determined using a PCR-based gene profiling approach of infected plant material for the presence or absence of all genes known to be required for the production of each alkaloid class. All alkaloid potential predictions, except for one, were validated by chemical analyses of infected plant material. Except for the gene responsible for the production of peramine in one of the FaTG-2 groups, variation in alkaloid gene content, specifically the presence and absence of genes, and copy number of gene clusters explained the chemotype (chemical phenotype) diversity observed in the tall fescue collection. The results from this study provide insight into endophyte germplasm and diversity present in tall fescue collections."
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"ISBN: 978-1-4675-4762-8"
Geographic Terms:Greece
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Young, C., and J. Takach. 2012. Understanding alkaloid diversity in tall fescue endophtyes [endophytes]. Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. p. 114.
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    Last checked: 09/06/2016
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