| |
| Web URL(s): | http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=115
Last checked: 09/06/2016
Requires: PDF Reader
Notes: Item is within a single large file |
|---|
| Publication Type:
| Report |
|---|
| Author(s): | Lee, Sang-Hoon |
|---|
| Author Affiliation: | National Institute of Animal Science, Cheonan, Chungnam, South Korea |
|---|
| Title: | Development of an antibiotic marker-free creeping bentgrass with resistance to two herbicides |
|---|
| Section: | Poster abstracts
Other records with the "Poster abstracts" Section
|
|---|
| Meeting Info.: | Salt Lake City, Utah: June 4-7, 2012 |
|---|
| Source: | Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. 2012, p. 115. |
|---|
| Publishing Information: | s.l.: s.n. |
|---|
| # of Pages: | 1 |
|---|
| Keywords: | TIC Keywords: Agrostis stolonifera; Genetic engineering; Herbicide resistance; Transgenic turfgrasses
|
|---|
| Abstract/Contents: | "Creeping bentgrass (Agrostis stolonifera L.) is one of the most widely used turf species in golf greens around the world. When a golf green is established, weeds hinder early growth and settlement of the grass, decreasing productivity in the green, and interfering with its later growth. To prevent the emergence of herbicide-resistant weeds when only one herbicide is in use, we sought to develop a new dual-resistance creeping bentgrass to facilitate mixture processing with much lower density or systematic approach through a combination of non-selective herbicides. Herbicide-resistant creeping bentgrass without antibiotic resistance markers were produced through Agrobacterium-mediated genetic transformation method. Embryogenic callus were infected with Agrobacterium tumefaciens EHA105 harboring the bar and the CP4-EPSPS genes for bialaphos and glyphosate resistance, respectively. Phosphinothricin-resistant calli and plants were selected. Soil-grown plants were obtained 14-16 weeks after transformation. Stable transformation of the selected regenerated plants was validated by PCR and Southern blot analysis revealed that at least one copy of transgene was integrated into the genome of each transgenic plants. Transgene expression was further confirmed by northern blot analysis and the CP4-EPSPS protein was detected by ELISA. Transgenic plants remained green and healthy when sprayed with Basta containing 0.5% glufosinate ammonium or glyphosate. Agrobacterium-mediated transformation efficiency was high (9.4%). For the first time herein, we report the Agrobacterium-mediated transformation of bentgrass with two herbicide-resistance genes. Thus, this effective and reliable method could be used as routine transformation and may facilitate the development of new varieties of creeping bentgrass." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| Note: | This item is an abstract only!
"ISBN: 978-1-4675-4762-8"
"MBFT2012" |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lee, S.-H. 2012. Development of an antibiotic marker-free creeping bentgrass with resistance to two herbicides. Proceedings of the 7th International Symposium on the Molecular Breeding of Forage and Turf. p. 115. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=274926 |
| If there are problems with this record, send us feedback about record 274926. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://www.grasslandoregon.com/assets/molecular-breeding-of-forage-and-turf.pdf#page=115
Last checked: 09/06/2016
Requires: PDF Reader
Notes: Item is within a single large file |
| Find from within TIC:
Digitally in TIC by file name: mbfat2012 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
