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Web URL(s): | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592366/ Last checked: 09/20/2016 Notes: Item is within a single large file http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592366/pdf/447.pdf#page=6 Last checked: 09/22/2016 Requires: PDF Reader Notes: Item is within a sinlge large file http://journals.fcla.edu/jon/article/view/81586/78715 Last checked: 08/17/2018 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Refereed |
Author(s): | Becker, J. Ole;
Witte, H.;
Becker, J. Smith;
Douhan, G. W.;
Vogel, J. P.;
Ploeg, A. |
Author Affiliation: | J. Ole Becker, Witte, J. Smith Becker, and Ploeg: Department of Nematology; Douhan: Department of Plant Pathology anfd Microbiology, University of California, Riverside; Vogel: Genomics and Gene Discovery Unit, USDA-ARS Western Regional Research Center, Albany, CA |
Title: | Brachypodium distachyon: A model plant to study root-knot nematode-pooideae interactions |
Section: | Abstracts Other records with the "Abstracts" Section
|
Source: | Journal of Nematology. Vol. 44, No. 4, December 2012, p. 452. |
Publishing Information: | Lawrence, Kansas: Society of Nematologists |
# of Pages: | 1 |
Keywords: | TIC Keywords: Brachypodium distachyon; Endophytic fungi; Meloidogyne graminis; Meloidogyne incognita; Nematode reproductivity
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Abstract/Contents: | "Brachypodium distachyon (purple false brome) is an annual grass of Mediterranean origin but worldwide distribution. It belongs to the grass subfamily Pooideae that includes cool season cereal crops, forage and turf grasses. With favorable characteristics such as small size, fast cycling, inbreeding, compact genome, and diverse diploid accessions, it has been championed as a model for cereal crops and biofuel grasses. We are investigating its research utility for studies with root-knot nematode species. Seeds of various inbred lines were imbibed in water and decontaminated in 10% bleach. After one week of moist incubation at 4°C, germinated seed was planted in sandy soil or soilless growth pouches. The growth media were infested with second-stage juveniles of either Meloidogyne incognita race 1 or M. graminis and incubated at 23±2°C in ambient light. In the growth pouches, slight root swellings were observed after 15-17 days and eventually the enlarging females broke through the root surface. Although egg production on the different B. distachyon lines were quite variable, most were better hosts for M. graminis than M. incognita with an average of about 300 eggs/egg mass and approximately 5 times higher egg population density than M. incognita based on root length. The first eggs of both species were observed in the growth pouches after 27 days. In many growth pouches fungal growth occurred that developed on plant material and produced abundant dark and hairy globose perithecia. The fungus was identified by ITS rDNA sequence analysis as Chaetomium globosum, a common endophyte and seedborne fungus associated with many plant species. Although no obvious effects on root-knot nematodes were observed, its potential influence on pathogen-host interactions requires further investigations." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Becker, J. O., H. Witte, J. S. Becker, G. W. Douhan, J. P. Vogel, and A. Ploeg. 2012. Brachypodium distachyon: A model plant to study root-knot nematode-pooideae interactions. J. Nematol. 44(4):p. 452. |
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| Web URL(s): http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592366/ Last checked: 09/20/2016 Notes: Item is within a single large file http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592366/pdf/447.pdf#page=6 Last checked: 09/22/2016 Requires: PDF Reader Notes: Item is within a sinlge large file http://journals.fcla.edu/jon/article/view/81586/78715 Last checked: 08/17/2018 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b2224870a |
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