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| Web URL(s): | https://scisoc.confex.com/scisoc/2016am/webprogram/Paper99879.html
Last checked: 11/22/2016 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Doherty, Joseph;
Crouch, Jo Anne;
Roberts, Joseph |
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| Author Affiliation: | Doherty and Roberts: Plant Science and Landscape Architecture, University of Maryland, College Park, MD; Crouch: Systematic Mycology & Microbiology, USDA-ARS, Beltsville, MD |
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| Title: | Elucidating the influence of seeds and soil on the developing creeping bentgrass microbiome |
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| Section: | Turfgrass breeding and genetics
Other records with the "Turfgrass breeding and genetics" Section
C05 turfgrass science
Other records with the "C05 turfgrass science" Section
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| Meeting Info.: | Phoenix, Arizona: November 6-9, 2016 |
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| Source: | ASA, CSSA and SSSA International Annual Meetings. 2016, p. 99879. |
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| Publishing Information: | [Milwaukee, Wisconsin]: [American Society of Agronomy and the Entomological Society of America] |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Cultivar evaluation; DNA extraction; Microbiomes; Soil microcosms; Soil sterilization
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| Cultivar Names: | 007 |
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| Abstract/Contents: | "The plant microbiome encompasses all microbial groups populating both the rhizo and phyllosphere. Research on microbiomes has increased in recent years, but our knowledge of microbial communities in turfgrasses is limited. The objective of this research is to investigate the impact resident seed and soil microbiota have on the development of the creeping bentgrass (Agrostis stolonifera cv. '007') microbiome over time. Creeping bentgrass was established in a modified laminar flow hood to create a sterile-air growing environment. Seeds were planted in 50 mL conical tubes of either sterilized or non-sterilized soil (85% sand 15% peat). Conical tubes of sterilized soils remained unplanted to serve as a control. Treatment replicates were arranged in a randomized complete block design and all received sterilized half-strength Hoaglands solution 2X weekly in addition to sterile dH2O applied daily. Samples of above and below ground tissue were taken at 2, 4, and 6 weeks post emergence. Total available DNA from plant tissues were extracted using Invitrogens EasyDNA kit and soil microbial DNA was extracted with the MoBio Power Soil DNA kit. Following extraction, DNA was barcoded and pooled, prior to analysis on the Illumina MiSeq platform. Metagenomic data was analyzed using the default pathway in QIIME and statistical analysis performed using the Vegan package in R. Turf planted in non-sterile soil emerged sooner and exhibited more vigor when compared to turf established in sterile soil. In addition, yield from metagenomic DNA extractions increased as turf matured. Moving forward long-term studies will be useful in assessing seasonal fluxes in microbiome populations." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related article "Elucidating the influence of resident seed and soil microbiota on the developing creeping bentgrass microbiome" Agrosystems, Geosciences & Environment, 3(1) 2020, p. e20038 [1-12], R=327541. R=327541 |
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| Note: | This item is an abstract only!
"242-3" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Doherty, J., J. A. Crouch, and J. Roberts. 2016. Elucidating the influence of seeds and soil on the developing creeping bentgrass microbiome. Agron. Abr. p. 99879. |
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Last checked: 11/22/2016 |
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