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| Web URL(s): | https://scisoc.confex.com/scisoc/2016am/webprogram/Paper100712.html
Last checked: 12/01/2016 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Edinger-Marshall, Susan B.;
Baston, David S.;
Lee, Christopher;
Yamamoto, Scott P. |
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| Author Affiliation: | Edinger-Marshall: Humboldt State University, Arcata, CA; Baston: College of Natural Resources and Sciences, Humboldt State University, Arcata, CA; Lee: CalFire, Fortuna, CA; Yamamoto: Humboldt State university, Arcata, CA |
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| Title: | Seasonal variation in isolation of phytophthora from wildland soils |
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| Section: | SSSA division: Soil biology and biochemistry
Other records with the "SSSA division: Soil biology and biochemistry" Section
Soil biology and biochemistry poster II
Other records with the "Soil biology and biochemistry poster II" Section
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| Meeting Info.: | Phoenix, Arizona: November 6-9, 2016 |
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| Source: | ASA, CSSA and SSSA International Annual Meetings. 2016, p. 100712. |
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| Publishing Information: | [Milwaukee, Wisconsin]: [American Society of Agronomy and the Entomological Society of America] |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Baiting; DNA extraction; Disease identification; Phytophthora; Polymerase chain reaction; Seasonal variation; Wildland/urban interface
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| Abstract/Contents: | "Phytophthora species are responsible for a variety of plant diseases including Sudden Oak Death (SOD) and Port Orford-cedar root disease. Phytophthora species were isolated from four soil/vegetation combinations in Northwestern California over four seasons for two consecutive years. Soils were sampled from oak woodlands, mixed-conifer forests on serpentine soils, mixed conifer soils on non-serpentine soils, and a moist control site known to have Phytophthora lateralis-infected Port Orford-cedar. We baited Phytophthora from the soil samples using rhododendron and Port Orford-cedar leaf baits. We isolated oomycete colonies growing from the baits on Phytophthora-selective PARPNH media and then transferred single hyphal tips to clarified V8 broth. We subsequently freeze-dried the individual colonies, extracted DNA, amplified the ITS region using the primer pair ITS 6F and ITS 4R, purified the PCR products, and sequenced them for species identification. Our data indicate we were most successful in baiting Phytophthora from soils collected during the spring period. BLAST searching of two Phytophthora-specific databases as well as Genbank showed that almost all Phytophthora isolated was P. cambivora; furthermore, it came exclusively from oak woodland soils. Mixed conifer, serpentine, and control soils yielded primarily other oomycetes, zygomycetes, and a Penicillium species that grew on the selective medium. Baiting and incubating samples in cold vs room-temperature conditions showed that room temperatures are necessary for Phytophthora culture growth, whereas Pythium species can survive and dominate in cold temperatures. We plan to continue our seasonal tracking of Phytophthora recovery from a variety of soil and forest types in northwestern California." |
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| Language: | English |
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| References: | 0 |
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| Note: | "466-408"
This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Edinger-Marshall, S. B., D. S. Baston, C. Lee, and S. P. Yamamoto. 2016. Seasonal variation in isolation of phytophthora from wildland soils. Agron. Abr. p. 100712. |
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Last checked: 12/01/2016 |
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