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| Web URL(s): | https://scisoc.confex.com/scisoc/2016am/webprogram/Paper102306.html
Last checked: 12/01/2016 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Bernstein, Rachael;
Roberts-Sano, Olivia;
DaCosta, Michelle;
Jung, Geunhwa;
Ebdon, Jeffery Scott |
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| Author Affiliation: | Bernstein: University of Massachusetts-Amherst, Amherst, MA; Roberts-Sano: University of Massachusetts-Amherst, Amherst, MA; DaCosta: University of Massachusetts, University of Massachusetts-Amherst, Amherst, MA; Jung: University of Massachusetts-Amherst, Amherst, MA; Ebdon: Stockbridge School of Agriculture, University of Massachusetts-Amherst, Amherst, MA |
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| Title: | Changes in gene expression in relation to freezing tolerance of perennial ryegrass |
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| Section: | C02 crop physiology and metabolism
Other records with the "C02 crop physiology and metabolism" Section
C-2/C4 graduate school oral competition - II
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| Meeting Info.: | Phoenix, Arizona: November 6-9, 2016 |
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| Source: | ASA, CSSA and SSSA International Annual Meetings. 2016, p. 102306. |
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| Publishing Information: | [Milwaukee, Wisconsin]: [American Society of Agronomy and the Entomological Society of America] |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Cold acclimation; Cold resistance; Cultivar evaluation; Gene expression; Lolium perenne; Overwintering
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| Abstract/Contents: | "Perennial ryegrass (Lolium perenne) is an economically important grass species used for turf and forage, but often exhibits poor winter survival compared to other cool-season grasses. A better understanding of the mechanisms required for freezing tolerance and overwintering capacity are needed to aid in the development of cultivars better adapted to northern climates. The objectives of the research were to examine changes in freezing tolerance and gene expression during cold acclimation and deacclimation for two perennial ryegrass genotypes different in freezing tolerance, described herein as freezing tolerant (TOL) and freezing sensitive (SENS) genotypes. Plants were exposed to five temperature treatments including: (1) non-acclimated control at 20°C for 2 weeks, (2) cold acclimated 2°C for 2 weeks, (3) cold acclimated -2°C for 2 weeks, (4) deacclimated at 4°C for 1d, (5) deacclimated at 4°C for 5 d. Following each sequential temperature treatment, leaves and crowns were harvested for evaluation of freezing tolerance (lethal temperature resulting in 50% mortality, LT50) and gene expression using qPCR. Genes were selected based on previous research demonstrating their importance in cold acclimation including CBF3, IRI-b, Prft1, IRI-a and PIP1. TOL genotypes exhibited higher acclimation capacity by maintaining higher freezing tolerance during cold acclimation and deacclimation. The expression of cold regulated genes among the genotypes will be discussed." |
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| Language: | English |
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| References: | 0 |
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| Note: | "94-6"
This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bernstein, R., O. Roberts-Sano, M. DaCosta, G. Jung, and J. S. Ebdon. 2016. Changes in gene expression in relation to freezing tolerance of perennial ryegrass. Agron. Abr. p. 102306. |
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Last checked: 12/01/2016 |
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