Full TGIF Record # 279675
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DOI:10.1094/PDIS-06-16-0834-RE
Web URL(s):http://apsjournals.apsnet.org/doi/full/10.1094/PDIS-06-16-0834-RE
    Last checked: 01/25/2017
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Publication Type:
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Refereed
Author(s):Villari, Caterina; Mahaffee, Walter F.; Mitchell, Thomas K.; Pedley, Kerry F.; Pieck, Michael L.; Hand, Francesca Peduto
Author Affiliation:Villari: Department of Plant Pathology, The Ohio State University, Columbus; Mahaffee: Horticultural Crops Reserach Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Corvallis, OR; Mitchell: Department of Plant Pathology, The Ohio State University; Pedley and Pieck: Foreign Disease-Weed Science Research Unit, USDA-ARS, Fort Detrick, MD; Hand: Department of Plant Pathology, The Ohio State University, Columbus, OH
Title:Early detection of airborne inoculum of Magnaporthe oryzae in turfgrass fields using a quantitative LAMP assay
Source:Plant Disease. Vol. 101, No. 1, January 2017, p. 170-177.
Publishing Information:[Washington, D.C.]: Bureau of Plant Industry, U.S. Dept. of Agriculture
# of Pages:8
Related Web URL:http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-06-16-0834-RE
    Last checked: 01/25/2017
    Notes: Abstract only
Keywords:TIC Keywords: Application timing; Diagnostic techniques; Disease distribution; Disease identification; Evaluations; Gray leaf spot; Lolium perenne; Pyricularia grisea; Quantitative techniques
Abstract/Contents:"Gray leaf spot (GLS) is a destructive disease of perennial ryegrass caused by a host specific pathotype of the ascomycete Magnaporthe oryzae. Early diagnosis is crucial for effective disease management and the implementation of Integrated Pest Management practices. However, a rapid protocol for the detection of low levels of airborne inoculum is still missing. We developed a pathogen-specific quantitative loop-mediated isothermal amplification (qLAMP) assay coupled with a spore trap system for rapid detection and quantification of airborne inoculum of the M. oryzae perennial ryegrass pathotype, and tested its suitability for implementation in GLS-infected turfgrass fields. In summer 2015, two perennial ryegrass plots were artificially inoculated with the pathogen, with four continuously running custom impaction spore traps placed in each plot. Sampling units were replaced daily and tested with the developed qLAMP assay, while plots were monitored for symptom development. Results confirmed that the qLAMP assay-trap system was able to detect as few as 10 conidia up to 12 days before symptoms developed in the field. LAMP technology is particularly appropriate for field implementation by nontechnical users, and has the potential to be a powerful decision support tool to guide timing of fungicide applications for GLS management."
Language:English
References:48
Note:Pictures, b/w
Figures
Tables
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Villari, C., W. F. Mahaffee, T. K. Mitchell, K. F. Pedley, M. L. Pieck, and F. P. Hand. 2017. Early detection of airborne inoculum of Magnaporthe oryzae in turfgrass fields using a quantitative LAMP assay. Plant Disease. 101(1):p. 170-177.
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DOI: 10.1094/PDIS-06-16-0834-RE
Web URL(s):
http://apsjournals.apsnet.org/doi/full/10.1094/PDIS-06-16-0834-RE
    Last checked: 01/25/2017
    Access conditions: Item is within a limited-access website
http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PDIS-06-16-0834-RE
    Last checked: 01/25/2017
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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