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Web URL(s): | http://cykx.lzu.edu.cn/EN/Y2013/V30/I12/1980 Last checked: 02/14/2017 Notes: Guide page |
Publication Type:
| Refereed |
Author(s): | Tian, Chen-xia;
Zhang, Yong-mei;
Ma, Hui-ling |
Author Affiliation: | Tian and Ma: Pratacultural College, Gansu Agricultural University, Lanzhou, China and Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, China and Sino-U. S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, China; Zhang: Instrumental Research & Analysis Center, Gansu Agricultural University, Lanzhou, China |
Title: | Primary study on preparation procedure of paraffin section with embryo structure of Poa pratensis |
Source: | [Caoye Kexue] [Pratacultural Science]. Vol. 30, No. 12, December 15 2013, p. 1980-1986. |
Publishing Information: | [Lanzhou Shi, China]: ["Cao Ye Ke Xue" Bian Jibu] |
# of Pages: | 7 |
Related Web URL: | http://cykx.lzu.edu.cn//EN/abstract/abstract10324.shtml Last checked: 02/08/2017 Notes: Abstract only |
Keywords: | TIC Keywords: Dye binding; Embryology; Paraffin section; Poa pratensis
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Abstract/Contents: | "A study was conducted to understand embryo structure of Poa pratensis with paraffin section. The effects of Sarranine-Fast green and Ehrlich's hematoxylin-eosin double stains with different dyeing time were analyses to improve the quality of paraffin section. This study selected better preparation procedure is as following: 95% eosin alcohol was selected instead of 95% alcohol for dehydration; multisegment dimethybrnzene was used instead of ethyl alcohol for transparent; rising temperature along with more wax gradually for wax dipping; slices were flatted and taken out in 40°C warm water bath and other improvements during procedure. Based on these improvements, better quality paraffin sections were achieved with dyeing with Ehrlich's hematoxylin-eosin double stains for 30 min and returning blue with water for 30 min." |
Language: | Chinese |
References: | 18 |
Note: | Abstract also appears in English Pictures, color Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Tian, C.-X., Y.-M. Zhang, and H.-l. Ma. 2013. Primary study on preparation procedure of paraffin section with embryo structure of Poa pratensis. (In Chinese) [Caoye Kexue] [Pratacultural Science]. 30(12):p. 1980-1986. |
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| Web URL(s): http://cykx.lzu.edu.cn/EN/Y2013/V30/I12/1980 Last checked: 02/14/2017 Notes: Guide page |
| MSU catalog number: b10275836 |
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