Full TGIF Record # 280635
Item 1 of 1
Web URL(s):http://cykx.lzu.edu.cn/EN/Y2013/V30/I12/1980
    Last checked: 02/14/2017
    Notes: Guide page
Publication Type:
i
Refereed
Author(s):Tian, Chen-xia; Zhang, Yong-mei; Ma, Hui-ling
Author Affiliation:Tian and Ma: Pratacultural College, Gansu Agricultural University, Lanzhou, China and Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, China and Sino-U. S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, China; Zhang: Instrumental Research & Analysis Center, Gansu Agricultural University, Lanzhou, China
Title:Primary study on preparation procedure of paraffin section with embryo structure of Poa pratensis
Source:[Caoye Kexue] [Pratacultural Science]. Vol. 30, No. 12, December 15 2013, p. 1980-1986.
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Ke Xue" Bian Jibu]
# of Pages:7
Related Web URL:http://cykx.lzu.edu.cn//EN/abstract/abstract10324.shtml
    Last checked: 02/08/2017
    Notes: Abstract only
Keywords:TIC Keywords: Dye binding; Embryology; Paraffin section; Poa pratensis
Abstract/Contents:"A study was conducted to understand embryo structure of Poa pratensis with paraffin section. The effects of Sarranine-Fast green and Ehrlich's hematoxylin-eosin double stains with different dyeing time were analyses to improve the quality of paraffin section. This study selected better preparation procedure is as following: 95% eosin alcohol was selected instead of 95% alcohol for dehydration; multisegment dimethybrnzene was used instead of ethyl alcohol for transparent; rising temperature along with more wax gradually for wax dipping; slices were flatted and taken out in 40°C warm water bath and other improvements during procedure. Based on these improvements, better quality paraffin sections were achieved with dyeing with Ehrlich's hematoxylin-eosin double stains for 30 min and returning blue with water for 30 min."
Language:Chinese
References:18
Note:Abstract also appears in English
Pictures, color
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tian, C.-X., Y.-M. Zhang, and H.-l. Ma. 2013. Primary study on preparation procedure of paraffin section with embryo structure of Poa pratensis. (In Chinese) [Caoye Kexue] [Pratacultural Science]. 30(12):p. 1980-1986.
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Web URL(s):
http://cykx.lzu.edu.cn/EN/Y2013/V30/I12/1980
    Last checked: 02/14/2017
    Notes: Guide page
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