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DOI: | 10.2135/cropsci2016.06.0462 |
Web URL(s): | https://dl.sciencesocieties.org/publications/cs/articles/57/supplement1/S-13 Last checked: 08/18/2017 Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/pdfs/57/supplement1/S-13 Last checked: 08/18/2017 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Mao, Qing;
Huff, David R. |
Author Affiliation: | Mao: Intercollege Graduate Degree Program in Plant Biology, Huck Institute of Life Sciences, Pennsylvania State Univ., University Park, PA and Dep. of Plant Science, Pennsylvania State Univ., University Park, PA and Complete Genomics, Inc., Mountain View, CA; Huff: Intercollege Graduate Degree Program in Plant Biology, Huck Institute of Life Sciences, Pennsylvania State Univ., University Park, PA and Dep. of Plant Science, Pennsylvania State Univ., University Park, PA |
Title: | Characterizing small RNA profiles in allotetraploid Poa annua L. and its diploid parents |
Section: | International Turfgrass Society Conference Other records with the "International Turfgrass Society Conference" Section
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Source: | Crop Science. Vol. 57, No. Supplement 1, July/August 2017, p. S-13-S-25. |
Publishing Information: | Madison, Wisconsin: Crop Science Society of America |
# of Pages: | 13 |
Related Web URL: | https://dl.sciencesocieties.org/publications/cs/abstracts/57/supplement1/S-13 Last checked: 08/18/2017 Notes: Abstract only https://dl.sciencesocieties.org/publications/cs/supplements/0/cropsci2016-06-0462_supp-figs_supplement1.pdf Last checked: 03/06/2017 Requires: PDF Reader Access conditions: Item is within a limited-access website Notes: Supplemental figures https://dl.sciencesocieties.org/publications/cs/supplements/0/cropsci2016-06-0462_supp-tables_supplement2.pdf Last checked: `3/06/2017 Requires: PDF Reader Access conditions: Item is within a limited-access website Notes: Supplemental tables |
Keywords: | TIC Keywords: Cultivar evaluation; Genetic analysis; Poa annua; Poa infirma; Poa supina; Polyploidy; Ribonucleic acid
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Abstract/Contents: | "A role for small RNAs has been implicated for polyploid evolution. This study was designed to examine the small RNA profiles of Poa annua L. and its diploid parental species. Four profiles, Poa infirma Kunth., Poa supina Schrad., perennial-type Poa annua, and annual-type Poa annua, were analyzed using three biological replicates representing each profile, resulting in a total of 12 libraries, totaling 20,920,659 small (1830 nucleotide) RNA sequences. Small RNA length distributions were compared between biological replicates within each profile, as well as between profiles. Both redundant and unique small RNA length distributions were similar among the four profiles (Cramer's V < 0.05 for overall profile comparison). A total of 121 conserved microRNAs belonging to 27 families were identified from the four profiles, and we did not observe specific patterns in expression levels of these microRNA families when comparing between these profiles. A total of 79 novel microRNAs were predicted using reference genomes from three model plant species. It was interesting to find that the perennial-type and annual-type Poa annua displayed very similar small RNA profiles, despite their morphological and life history differences. In addition, the small RNA profiles of tetraploid Poa annua were very similar to its diploid parental species as well. The incorporation of biological replicates in our study helped to increase our confidence in the results of our data analyses." |
Language: | English |
References: | 45 |
Note: | Figures Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Mao, Q., and D. R. Huff. 2017. Characterizing small RNA profiles in allotetraploid Poa annua L. and its diploid parents. Crop Sci. 57(Supplement 1):p. S-13-S-25. |
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| DOI: 10.2135/cropsci2016.06.0462 |
| Web URL(s): https://dl.sciencesocieties.org/publications/cs/articles/57/supplement1/S-13 Last checked: 08/18/2017 Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/pdfs/57/supplement1/S-13 Last checked: 08/18/2017 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: b2211522a |
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