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| Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/1993jou768.pdf
Last checked: 02/02/2009
Requires: PDF Reader |
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| Access Restriction: | Certain MSU-hosted archive URLs may be restricted to legacy database members. |
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| Publication Type:
| Refereed |
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| Author(s): | Sweeney, P. M.;
Danneberger, T. K.;
Kamalay, J. C. |
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| Author Affiliation: | Ohio State; USDA |
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| Title: | Fidelity of amplification fragment length polymorphisms (AFLPs) within multiple extractions from single seedlings of hard, chewings and red fescue |
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| Meeting Info.: | 7th International Turfgrass Society Research Conference, Palm Beach, FL, USA, 18-24 July, 1993 |
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| Source: | International Turfgrass Society Research Journal. Vol. 7, 1993, p. 768-774. |
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| Publishing Information: | Overland Park, KS: INTERTEC Publishing Corp. |
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| # of Pages: | 7 |
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| Keywords: | TIC Keywords: Amplification fragment length polymorphisms; DNA; Festuca ovina subsp. duriuscula; Festuca rubra subsp. commutata; Festuca rubra subsp. rubra; Genetic analysis; Polymerase chain reaction
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| Abstract/Contents: | "Polymerase chain reaction (PCR) amplification of DNA fragments using arbitrary oligonucleotide primers to generate AFLP's or random amplified polymorphic DNA (RAPD) shows promise as a method to aid in the genotypic characterization of turfgrass. Application of this method to heterogeneous populations, such as synthetic turfgrass cultivars, has not been evaluated. The study of population dynamics using genetic characterization of individuals would require a simple, fast procedure to extract DNA. The object of this study was to evaluate a fast DNA extraction method for use in PCR with arbitrary primers, and to determine the best method to assay PCR amplification products. To determine the fidelity of the extraction, PCR was used to amplify 3 separate extracts from a single plant of each species examined. The triplicate extracts consistently produced the same amplification products, and replication of the PCR amplification using the same DNA extracts produced identical amplified fragment patterns. Amplified products were separated via electrophoresis in agarose gels stained with ethidium bromide (EtBr) and silver stained polyacrylamide gels to determine the best method of resolving and documenting AFLP's. The results indicated that the crude DNA extracted via a fast extraction procedure was reliably amplified in PCR using arbitrary primers. Results also showed that PCR products were adequately assayed using agarose gels stained with EtBr." |
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| Language: | English |
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| References: | 18 |
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| Note: | "Chapter 109"
Figures |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sweeney, P. M., T. K. Danneberger, and J. C. Kamalay. 1993. Fidelity of amplification fragment length polymorphisms (AFLPs) within multiple extractions from single seedlings of hard, chewings and red fescue. Int. Turfgrass Soc. Res. J. 7:p. 768-774. |
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| Web URL(s):
https://archive.lib.msu.edu/tic/its/articles/1993jou768.pdf
Last checked: 02/02/2009
Requires: PDF Reader |
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MSU catalog number: SB 433 .I522 v.7 |
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