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| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2017.pdf#page=35
Last checked: 03/23/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Di, Rong;
Bonos, Stacy |
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| Author Affiliation: | Di: Department of Plant Biology, Rutgers University; Bonos: Department of Plant Biology, Rutgers University, New Brunswick, NJ |
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| Title: | CRISPR - Gene editing of creeping bentgrass to improve stress tolerance and disease resistance |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, New Jersey: January 13, 2017 |
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| Source: | Proceedings of the Twenty-Sixth Annual Rutgers Turfgrass Symposium. Vol. 26, 2017, p. 35. |
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| Publishing Information: | New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Clones; Disease resistance; Dollar spot; Expressed sequence tag; Gene isolation; Stress tolerance
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| Cultivar Names: | Crenshaw |
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| Abstract/Contents: | "Creeping bentgrass is one of the most widely used cool-season grass species on golf courses. However, there is not one cultivar of creeping bentgrass that is considered completely resistant to dollar spot disease caused by Sclerotinia homoeocarpa F.T. Bennet. Creeping bentgrass is also stressed by heat and drought during summer months. In this project, we use the CRISPR/Cas (clustered regularly interspaced short palindromic repeats-associated endonuclease) -gene editing technology to knock-out genes involved in disease susceptibility and negative stress regulation in creeping bentgrass to improve disease resistance and stress tolerance. Using bioinformatics tools, we have identified EST (expressed sequence tag) sequences for the following three genes that are related to disease and stress susceptibility in creeping bentgrass Crenshaw cultivar: BONL (BONZAI1-like), CPKL (calcium-dependent protein kinase 12-like) and DREBL (dehydration responsive element binding 1c-like). We have cloned the partial cDNAs of these three genes from Crenshaw creeping bentgrass by RT-PCR (reverse transcription -polymerase chain reaction). We have also cloned and sequenced partial genomic DNA (gDNA) sequences of these three genes from Crenshaw. The gene target sites have been identified in these genes gDNA sequences, and the CRISPR-editing vectors containing the gRNA (guide RNA) sequences and the Cas9 nuclease cassettes have been constructed. We have developed the tissue culture system for Crenshaw creeping bentgrass transformation, and the CRISPR-gene editing vectors have been transformed into callus tissues by gene gun bombardment. The CRISPR-gene editing platform is being extended into other turfgrass species." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Di, R., and S. Bonos. 2017. CRISPR - Gene editing of creeping bentgrass to improve stress tolerance and disease resistance. Proc. Rutgers Turfgrass Symp. 26:p. 35. |
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| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2017.pdf#page=35
Last checked: 03/23/2017
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b3696858 |
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