| |
| DOI: | 10.1094/PDIS-09-16-1385-PDN |
|---|
| Web URL(s): | http://apsjournals.apsnet.org/doi/full/10.1094/PDIS-09-16-1385-PDN
Last checked: 04/10/2017 |
|---|
| Publication Type:
| Report |
|---|
| Author(s): | Hu, J.;
Liu, Q. Y.;
Liu, M. H.;
Zhang, F. G.;
Xiao, Y.;
Niu, C. |
|---|
| Author Affiliation: | Hu, Q. Y. Liu, M. H. Liu, Zhang, and Xiao: College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, China; Niu: Beijing Cs Forturf Agri Tech Co., Ltd, Beijing, China |
|---|
| Title: | First report of summer patch of creeping bentgrass caused by Magnaporthe poae in China |
|---|
| Section: | Disease notes: Diseases caused by fungi and fungus-like organisms
Other records with the "Disease notes: Diseases caused by fungi and fungus-like organisms" Section
|
|---|
| Source: | Plant Disease. Vol. 101, No. 4, April 2017, p. 634. |
|---|
| Publishing Information: | [Washington, D.C.]: Bureau of Plant Industry, U.S. Dept. of Agriculture |
|---|
| # of Pages: | 1 |
|---|
| Keywords: | TIC Keywords: Agrostis stolonifera; Disease development; Disease identification; Magnaporthe poae; Summer patch
|
|---|
| Cultivar Names: | T1 |
|---|
| Geographic Terms: | China |
|---|
| Abstract/Contents: | "Creeping bentgrass (Agrostis stolonifera L.) is an important cool-season perennial grass frequently used on golf courses in the north region of China. In late July 2016, disease symptoms occurred on creeping bentgrass putting greens in Beijing, after a strong rainfall and daily high air temperatures above 33°C. Symptoms appeared in tan, irregular, 20 to 50 cm diameter patches intermixed with both healthy and symptomatic turfgrass, which exhibited chlorosis and foliar dieback. Necrotic roots were frequently observed in diseased areas and colonized with ectotrophic hyphae based on microscopic observation. Similar symptoms and signs have been reported on creeping bentgrass caused by Magnaporthe poae Landschoot & Jackson on golf courses in North Carolina (Tredway 2005), and M. poae was recently confirmed to cause summer patch disease on Kentucky bluegrass in Beijing (Liu et al. 2016). Over 20 diseased samples from 'T1' creeping bentgrass putting greens at three golf courses were collected, dark root tips were cut, surface sterilized in 0.6% sodium hypochlorite (NaClO) for 5 min, washed twice with sterilized water, air dried for 1 min, and placed on potato dextrose agar (PDA) containing 50 mg liter-1 each of ampicillin, streptomycin sulfate, and tetracycline. Plates were incubated in the dark at room temperature for 2 to 4 days. A total of 15 fungal isolates with similar morphology (mycelium is hyaline initially, then becomes gray and finally appears black or olivaceous brown) were consistently recovered (Clarke and Gould 1993). DNA was extracted and amplified with primers ITS1F/ITS4 (White et al. 1990). PCR products were sequenced (deposited as KX775953 and KX775954 in GenBank) and showed 100% similarity to M. poae (accession no. KX256159) through BLAST analysis. To complete Kochs postulates, sterile soil was put into six plastic pots (15 cm height x 15 cm top diameter x 10 cm bottom diameter, three replicates for each isolate), 25 mg M. poae-infested creeping bentgrass seed was added and then covered by 2-month-old 'T1' creeping bentgrass transferred from the same size of plastic pots described above. Noninfested creeping bentgrass seeds were inoculated on healthy creeping bentgrass as controls. Pots were placed in a growth chamber with a 12-h day/night cycle of 35/28°C and 90% relative humidity. Disease symptoms (foliar dieback and necrotic roots) were noted 2 to 3 weeks after inoculation. M. poae was recovered from the roots of inoculated turf and identified morphologically and molecularly as described above. To our knowledge, this is the first report of summer patch on creeping bentgrass caused by M. poae in China. This research is an important step toward the development of management strategies for golf course superintendents in China." |
|---|
| Language: | English |
|---|
| References: | 4 |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hu, J., Q. Y. Liu, M. H. Liu, F. G. Zhang, Y. Xiao, and C. Niu. 2017. First report of summer patch of creeping bentgrass caused by Magnaporthe poae in China. Plant Disease. 101(4):p. 634. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=282573 |
| If there are problems with this record, send us feedback about record 282573. |
| Choices for finding the above item: |
|---|
| DOI: 10.1094/PDIS-09-16-1385-PDN |
| Web URL(s):
http://apsjournals.apsnet.org/doi/full/10.1094/PDIS-09-16-1385-PDN
Last checked: 04/10/2017 |
| 
MSU catalog number: b2191617 |
| Find from within TIC:
Physical sequential file in TIC. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
