Full TGIF Record # 285835
Item 1 of 1
DOI:10.3724/SP.J.1006.2014.01572
Web URL(s):http://zwxb.chinacrops.org/EN/article/downloadArticleFile.do?attachType=PDF&id=5717
    Last checked: 06/20/2017
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http://zwxb.chinacrops.org/article/2014/0496-3490-40-9-1572.html
    Last checked: 06/20/2017
Publication Type:
i
Refereed
Author(s):Feng, Xun-wei; Cai, Hong-wei
Author Affiliation:Feng and Cai: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China; Feng: Patent Examination Cooperation Center of the Patent Office, SIPO, Beijing, China
Title:Cloning of zoysiagrass CBF gene and validation of cold tolerance in transgenic Arabidopsis
Source:Acta Agronomica Sinica. Vol. 40, No. 9, 2014, p. 1572-1578.
Publishing Information:Beijing, China: Zhongguo Zuowu Xuehui [Crop Science Society of China]
# of Pages:7
Keywords:TIC Keywords: Arabidopsis; Clones; Cold resistance; Evaluations; Transgenic turfgrasses; Zoysia
Abstract/Contents:"Zoysiagrass is recognized as an excellent warm-season turfgrass and mainly used in subtropical and tropical regions. Cold stress is a major constraint factor for the cultivation of zoysiagrass. In this study, according to the sequences of cold tolerance gene CBF had been reported in other plant species, we cloned the corresponding homologous of the ZjCBF gene by homology cloning method in Zoysia japonica using a material originated from the most northern area of Japan. Based on the alignment results compared with other reported CBF genes, we found the ZjCBF gene belongs to the CBF1 familiy. By semi-quantitative PCR and real-time quantitative PCR, we analyzed the expression level of the ZjCBF gene in the cold condition and found that ZjCBF was induced by cold stress, and the ZjCBF expression reached peak at six hours post 4°C treatment. In addition, we also constructed ZjCBF over expression vector and generated transgenic Arabidopsis plants, with better cold tolerance than the wild-type, whether through cold acclimation or not."
Language:Chinese
References:17
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Abstract also appears in English
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Feng, X.-w., and H.-w. Cai. 2014. Cloning of zoysiagrass CBF gene and validation of cold tolerance in transgenic Arabidopsis. (In Chinese) Acta Agronomica Sinica. 40(9):p. 1572-1578.
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DOI: 10.3724/SP.J.1006.2014.01572
Web URL(s):
http://zwxb.chinacrops.org/EN/article/downloadArticleFile.do?attachType=PDF&id=5717
    Last checked: 06/20/2017
    Requires: PDF Reader
http://zwxb.chinacrops.org/article/2014/0496-3490-40-9-1572.html
    Last checked: 06/20/2017
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