Full TGIF Record # 287819
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DOI:10.2134/itsrj2016.10.0838
Web URL(s):https://dl.sciencesocieties.org/publications/its/articles/13/1/466
    Last checked: 10/11/2019
    Access conditions: Item is within a limited-access website
Publication Type:
i
Refereed
Author(s):Warnke, Scott E.; Thammina, Chandra S.; Amundsen, Keenan; Miljanic, Philip; Hershman, Haley
Author Affiliation:Warnke and Miljanic: Floral and Nursery Plants Research Unit, US National Arboretum, USDA-ARS, Beltsville, MD; Thammina: Floral and Nursery Plants Research Unit, US National Arboretum, USDA-ARS, Beltsville, MD and Dep. of Plant Biology and Pathology, Rutgers Univ., New Brunswick, NJ; Amundsen: Dep. of Agronomy and Horticulture, Univ. of Nebraska, Lincoln, NE; Hershman: Ohio Univ.
Title:High-resolution melt analysis of simple sequence repeats for bentgrass species differentiation
Section:Genetics and breeding
Other records with the "Genetics and breeding" Section
Meeting Info.:New Brunswick, New Jersey: July 16-21, 2017
Source:International Turfgrass Society Research Journal. Vol. 13, 2017, p. 1-5.
Publishing Information:s.l.: International Turfgrass Society
# of Pages:5
Keywords:TIC Keywords: Agrostis stolonifera; Agrostis tenuis; Comparisons; Cultivar evaluation; Cultivar variation; Differentiation; Interspecific hybrids; Simple sequence repeats
Abstract/Contents:"Agrostis stolonifera L. (creeping bentgrass) and Agrostis capillaris L. (colonial bentgrass) are turfgrass species well adapted for golf course use in regions of the world where cool-season grasses are grown. Interspecific hybrids between the species do form and have the potential to incorporate some of the beneficial characteristics of both species. Agrostis stolonifera has excellent quality at low mowing heights and recovers well from damage. Agrostis capillaris tends to exhibit more drought tolerance and a higher level of resistance to the common fungal pathogen Sclerotinia homoeocarpa. Fast and inexpensive methods of species differentiation could help seed certification agencies and enhance interspecific hybrid bentgrass development. Ninety-six simple sequence repeat primer pairs were designed from Roche 454 sequencing of A. stolonifera and A. capillaris genomic DNA and were tested for their potential for bentgrass species differentiation. Real-time polymerase chain reaction followed by high-resolution melt analysis was tested for its potential to speed up analysis and lower costs. Simple sequence repeat primer pairs were identified that can differentiate bentgrass species."
Language:English
References:17
Note:TIC-hosted web link available 2 years after publication date.
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Warnke, S. E., C. S. Thammina, K. Amundsen, P. Miljanic, and H. Hershman. 2017. High-resolution melt analysis of simple sequence repeats for bentgrass species differentiation. Int. Turfgrass Soc. Res. J. 13:p. 1-5.
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DOI: 10.2134/itsrj2016.10.0838
Web URL(s):
https://dl.sciencesocieties.org/publications/its/articles/13/1/466
    Last checked: 10/11/2019
    Access conditions: Item is within a limited-access website
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