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DOI: | 10.1080/00275514.2016.1277469 |
Web URL(s): | http://www.tandfonline.com/doi/pdf/10.1080/00275514.2016.1277469 Last checked: 08/15/2017 Requires: PDF Reader Access conditions: Item is within a limited-access website http://www.tandfonline.com/doi/full/10.1080/00275514.2016.1277469 Last checked: 08/08/2017 Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Zhang, Wei;
Card, Stuart D.;
Mace, Wade J.;
Christensen, Michael J.;
McGill, Craig R.;
Matthew, Cory |
Author Affiliation: | Zhang: Institute of Agriculture and Environment, Massey University, Palmerston North, New Zealand and Forage Improvement, AgResearch Ltd., Grasslands Research Centre, Palmerston North, New Zealand; Card, Mace, and Christensen: Forage Improvement, AgResearch Ltd., Grasslands Research Centre, Palmerston North, New Zealand; McGill and Matthew: Institute of Agriculture and Environment, Massey University, Palmerston North, New Zealand; Matthew: Institute of Agriculture and Environment, Massey University, Palmerston North, New Zealand |
Title: | Defining the pathways of symbiotic Epichloë colonization in grass embryos with confocal microscopy |
Section: | Cell and molecular biology Other records with the "Cell and molecular biology" Section
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Source: | Mycologia. Vol. 109, No. 1, 2017, p. 153-161. |
Publishing Information: | Lancaster, Pennsylvania: New Era Print Co. for the New York Botanical Garden |
# of Pages: | 9 |
Related Web URL: | http://www.tandfonline.com/doi/abs/10.1080/00275514.2016.1277469 Last checked: 08/15/2017 Notes: Abstract only |
Keywords: | TIC Keywords: Confocal microscopy; Embryos (plant); Endophytes; Epichloe; Evaluations; Festuca arundinacea; Fungus infection; Fungus profile; Seed production; Transmission
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Abstract/Contents: | "Asexual cool-season grass endophytes of the genus Epichlo¨e (Ascomycota: Clavicipitaceae) are strictly vertically disseminated. The hosts of these mutualistic fungi express no symptoms during the fungal lifecycle that takes place entirely within the plant, while their hosts receive beneficial outcomes. These fungi are distributed in two major locations within the mature seeds of their hosts; namely, within the embryo (including the scutellum, coleoptile, plumule, radicle, and coleorhiza tissues) and between the aleurone and pericarp layers, with the latter hyphae playing no role in transmission of the fungus to the next plant generation. Conflicting evidence remains in the literature on the timing of embryo colonization. In a detailed investigation, utilizing confocal microscopy to observe the distribution of Epichloë coenophiala strain AR601 in tall fescue (Lolium arundinaceum), we tracked endophyte hyphal colonization in the ovary (pre-fertilization) through to the fully mature seed stage. Confocal microscopy images revealed that at the early and mature developmental stages of the embryo sac, before host grass fertilization, there were large quantities of endophyte mycelium present, especially around the antipodal cells, indicating that this endophyte enters the embryo sac before the fertilization stage. After host fertilization, fungal hyphae could be seen in the true embryo and early nonstarchy endosperm. Understanding the mechanisms of transmission to the seed is important for commercial seed producers and end users." |
Language: | English |
References: | 47 |
Note: | Pictures, color |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Zhang, W., S. D. Card, W. J. Mace, M. J. Christensen, C. R. McGill, and C. Matthew. 2017. Defining the pathways of symbiotic Epichloë colonization in grass embryos with confocal microscopy. Mycologia. 109(1):p. 153-161. |
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| DOI: 10.1080/00275514.2016.1277469 |
| Web URL(s): http://www.tandfonline.com/doi/pdf/10.1080/00275514.2016.1277469 Last checked: 08/15/2017 Requires: PDF Reader Access conditions: Item is within a limited-access website http://www.tandfonline.com/doi/full/10.1080/00275514.2016.1277469 Last checked: 08/08/2017 Access conditions: Item is within a limited-access website |
| MSU catalog number: b2214983 MSU catalog number: b5343430 |
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