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| DOI: | 10.1016/j.soilbio.2017.06.006 |
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| Web URL(s): | http://www.sciencedirect.com/science/article/pii/S0038071717301888
Last checked: 08/09/2017
Access conditions: Item within a limited-access website 11/03/2015 |
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| Publication Type:
| Refereed |
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| Author(s): | Guo, Meixia;
Gong, Zongqiang;
Miao, Renhui;
Rookes, James;
Cahill, David;
Zhuang, Jie |
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| Author Affiliation: | Guo: Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, PR China and International Joint Research Laboratory for Global Change Ecology, College of Life Sciences, Henan University, Kaifeng, Henan, PR China; Gong: Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, PR China; Miao: International Joint Research Laboratory for Global Change Ecology, College of Life Sciences, Henan University, Kaifeng, Henan, PR China; Rookes and Cahill: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia; Zhuang: Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, PR China and Department of Biosystems Engineering and Soil Science, The University of Tennessee, Knoxville, TN |
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| Title: | Microbial mechanisms controlling the rhizosphere effect of ryegrass on degradation of polycyclic aromatic hydrocarbons in an aged-contaminated agricultural soil |
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| Source: | Soil Biology & Biochemistry. Vol. 113, October 2017, p. 130-142. |
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| Publishing Information: | Amsterdam, The Netherlands: Elsevier |
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| # of Pages: | 13 |
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| Keywords: | TIC Keywords: Analytical methods; Biodegradation; Denaturing gradient gel electrophoresis; Gene expression; HPLC; Lolium; Microbial activity; Microbiological soil analysis; Phytoremediation; Polycyclic aromatic hydrocarbons; Rhizosphere
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| Abstract/Contents: | "The phytoremediation of polycyclic aromatic hydrocarbons (PAHs) depends on plant-microbe interactions in the rhizosphere, but the extent and mechanisms of these effects are still unclear. The aim of this study was to investigate the effects of plants on PAHs degradation and on associated changes in transcriptionally active bacterial community structures, degrading bacteria, and especially functional gene expression in a PAH-contaminated agricultural soil. We conducted a rhizo-box experiment planted with ryegrass for 60 days under glasshouse conditions. The degradation of 12PAHs in rhizosphere and non-rhizosphere soils during the experiment was quantified using HPLC analysis. The dynamics of active bacterial community structure in soil were assessed using the molecular tool of cDNA-based denaturing gradient gel electrophoresis (DGGE). Functional PAH-ring hydroxylating dioxygenase (PAH-RHDĪ±) genes were cloned using the Gram-positive (GP) and Gram-negative (GN) primers. The abundance and activity of total bacteria and GP/GN PAH-degraders were monitored by real-time PCR. Results demonstrate that the agricultural soil contained known PAH degraders and RHDĪ± genes, such as nidA3, pdoA, nahAc and phnAc. The presence of ryegrass promoted the dissipation of PAHs and changed the structures of active bacterial communities in soil. In general, the abundance of total bacteria and GP PAH-degrader increased during the inoculation time. The ryegrass root enhanced the functional bacterial diversity in the early stages (0-10 days). However, the effect of ryegrass roots may stimulate the expression of GP and GN PAH-RHDĪ± genes after 40 days. Our results indicate that ryegrass increased the degradation of PAHs by promoting bacteria diversity, increasing the abundance of total bacteria and PAH degraders, and stimulating RHDĪ± gene expression." |
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| Language: | English |
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| References: | 69 |
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| Note: | Pictures, b/w
Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Guo, M., Z. Gong, R. Miao, J. Rookes, D. Cahill, and J. Zhuang. 2017. Microbial mechanisms controlling the rhizosphere effect of ryegrass on degradation of polycyclic aromatic hydrocarbons in an aged-contaminated agricultural soil. Soil Biol. Biochem. 113:p. 130-142. |
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| DOI: 10.1016/j.soilbio.2017.06.006 |
| Web URL(s):
http://www.sciencedirect.com/science/article/pii/S0038071717301888
Last checked: 08/09/2017
Access conditions: Item within a limited-access website 11/03/2015 |
| 
MSU catalog number: b2217194 |
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