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| DOI: | 10.1002/ps.4675 |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1002/ps.4675/full
Last checked: 10/05/2017
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1002/ps.4675/epdf
Last checked: 10/05/2017
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Laforest, Martin;
Soufiane, Brahim;
Simard, Marie-Josée;
Obeid, Kristen;
Page, Eric;
Nurse, Robert E. |
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| Author Affiliation: | Laforest, Soufiane, and Simard: Agriculture and Agri-Food Canada (AAFC), Saint-Jean-sur-Richelieu Research and Development Centre, St-Jean-sur-Richelieu, Québec, Canada; Obeid: Food and Rural Affairs, Harrow Research and Development Centre, Ontario Ministry of Agriculture, Harrow, Ontario, Canada; Page and Nurse: Agriculture and Agri-Food Canada (AAFC), Harrow Research and Development Centre, Harrow, Ontario, Canada |
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| Title: | Acetyl-CoA carboxylase overexpression in herbicide-resistant large crabgrass (Digitaria sanguinalis) |
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| Source: | Pest Management Science. Vol. 73, No. 11, November 2017, p. 2227-2235. |
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| Publishing Information: | Barking, Essex, United Kingdom: Elsevier Science Publishers Ltd. |
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| # of Pages: | 9 |
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| Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1002/ps.4675/abstract
Last checked: 10/05/2017
Notes: Abstract only |
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| Keywords: | TIC Keywords: Acetyl-CoA carboxylase inhibitors; Digitaria sanguinalis; Evaluations; Gene expression; Genetic analysis; Herbicide resistance; Weed biotypes; Weed control
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| Abstract/Contents: | "BACKGROUND: The occurrence of herbicide-resistant weed biotypes is increasing and this report of an acetyl-CoA carboxylase (ACCase) inhibitor-resistant Digitaria sanguinalis L. Scop. from southwestern Ontario is another example. The identified weed escaped control in an onion and carrot rotation in which graminicides were used for several consecutive years. Our goal was to characterize the level and mechanism of resistance of the biotype. RESULTS: The biotype was resistant to all five ACCase inhibitor herbicides tested. Gene-expression profiling was performed because none of the mutations known to confer resistance in the ACCase gene were detected. RNASeq and quantitative reverse-transcriptase PCR (qRT-PCR) results indicated that transcription of ACCase was 3.4-9.3 times higher in the resistant biotype than the susceptible biotype. ACCase gene copy number was determined by qPCR to be five to seven times higher in the resistant compared with the susceptible biotype. ACCase gene overexpression was directly related to the increase of the ACCase gene copy number. CONCLUSION: Our results are consistent with the hypothesis that overexpression of the herbicide target gene ACCase confers resistance to the herbicide. This is the first reported case of target gene duplication conferring resistance to a herbicide other than glyphosate." |
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| Language: | English |
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| References: | 48 |
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| Note: | Pictures, b/w
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Laforest, M., B. Soufiane, M.-J. Simard, K. Obeid, E. Page, and R. E. Nurse. 2017. Acetyl-CoA carboxylase overexpression in herbicide-resistant large crabgrass (Digitaria sanguinalis). Pest Management Science. 73(11):p. 2227-2235. |
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| DOI: 10.1002/ps.4675 |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1002/ps.4675/full
Last checked: 10/05/2017
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1002/ps.4675/epdf
Last checked: 10/05/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2219665 |
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