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| Web URL(s): | https://scisoc.confex.com/crops/2017am/webprogram/Paper108824.html
Last checked: 10/09/2017 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Wang, Ruying;
Clarke, Bruce B.;
Belanger, Faith C. |
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| Author Affiliation: | Wang and Clarke: Rutgers University, New Brunswick, NJ; Belanger: Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ |
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| Title: | CRISPR-Cas9 knockout of the Epichloë festucae antifungal protein gene |
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| Section: | C05 Turfgrass Science
Other records with the "C05 Turfgrass Science" Section
Turf science and management general poster (includes student competition)
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| Meeting Info.: | Tampa, Florida: October 22-25, 2017 |
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| Source: | ASA, CSSA and SSSA International Annual Meetings. 2017, p. 108824. |
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| Publishing Information: | [Milwaukee, Wisconsin]: [American Society of Agronomy and the Entomological Society of America] |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Fungicidal properties; Disease resistance; Disease susceptibility; Epichloe festucae; Festuca rubra subsp. rubra; Sclerotinia homoeocarpa
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| Abstract/Contents: | "Strong creeping red fescue (Festuca rubra subsp. rubra) is a commercially important low-maintenance turfgrass and is often naturally infected with the fungal endophyte Epichloë festucae. Endophyte-mediated disease resistance to the fungal pathogen Sclerotinia homoeocarpa F.T. Bennett, the causal agent of dollar spot disease, has been observed in the field. In a previous quantitative transcriptome study of the Epichloë festucae-strong creeping red fescue interaction, the second most abundant fungal transcript was found to encode a protein similar to antifungal proteins from Penicillium and Aspergillus species. This E. festucae gene is designated Efe-afpA and the protein is designated Efe-AfpA. The protein has been shown to inhibit the growth of S. homoeocarpa in plate assays. To further study the role of this protein in the endophyte-mediated disease resistance, we generated a gene knockout of Efe-afpA using the CRISPR-Cas9 system. This gene was disrupted by an insertion of a fragment from the Cas9 vector at the cutting site of Cas9 endonuclease. This was confirmed by PCR and sequencing. Next, the knockout isolate will be reintroduced into endophyte-free strong creeping red fescue to evaluate susceptibility of the host plant to dollar spot disease using an established greenhouse assay." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only!
"515" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, R., B. B. Clarke, and F. C. Belanger. 2017. CRISPR-Cas9 knockout of the Epichloë festucae antifungal protein gene. Agron. Abr. p. 108824. |
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Last checked: 10/09/2017 |
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