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| Web URL(s): | https://scisoc.confex.com/crops/2017am/webprogram/Paper108215.html
Last checked: 10/12/2017 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Wang, Kehua;
Dai, Xiaoxia;
Shi, Tianran;
Tian, Jinli;
Zhang, Wanjun |
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| Author Affiliation: | Wang: Department of Grassland Sciences, China Agricultural University, Beijing, Beijing, China; Dai, Shi, Tian, and Zhang: Department of Grassland Sciences, China Agricultural University, Beijing, China |
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| Title: | Overexpression of rice microRNA319 improves thermotolerance of perennial ryegrass |
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| Section: | C05 Turfgrass Science
Other records with the "C05 Turfgrass Science" Section
Turf physiology, breeding and genetics
Other records with the "Turf physiology, breeding and genetics" Section
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| Meeting Info.: | Tampa, Florida: October 22-25, 2017 |
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| Source: | ASA, CSSA and SSSA International Annual Meetings. 2017, p. 108215. |
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| Publishing Information: | [Milwaukee, Wisconsin]: [American Society of Agronomy and the Entomological Society of America] |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Antioxidant metabolism; Genetic analysis; Heat resistance; Heat stress; Lolium perenne; Ribonucleic acid; Stress response; Transgene expression
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| Abstract/Contents: | "Heat stress is a major problem affecting the growth of cool-season turfgrasses in transition zone and warmer regions, e.g. perennial ryegrass (Lolium perenne L.). MicroRNA319 (miR319) was found to be involved in both plant development and plant responses to various abiotic stresses, including drought, salinity, and cold. However, it is unclear if overexpression of miR319 affects plant heat tolerance. And our greenhouse observation found miR319 transgenic perennial ryegrass plants seemed to have better thermo-tolerance than WT plants. Our objective was to investigate if the constitutive expression of miR319 could enhance the thermo-tolerance in perennial ryegrass and the possible physiological and molecular mechanism underneath. Our results showed that overexpression of miR319 gene resulted in wider leaf blades in perennial ryegrass. Moreover, under both short-term (27 h, 42 ° C) and long-term (9 day, 35 ° C) high temperature treatments, transgenic perennial ryegrass plants showed enhanced heat stress tolerance compared to the wild type plants as manifested by better grass quality, lower shoot electrolyte leakage (50% lower than that of wild type plants), and higher relative leaf water content. Whole genome transcriptional analysis found that some genes related to antioxidative system, stress responsive transcription factors (NAC, HSF, e.g.) and Jasmonic acid biosynthesis were altered in transgenic plants under both control and heat stress conditions. In a summary, our findings of the study demonstrate that miR319 plays an important role in transgenic perennial ryegrass response to heat stress, which may result from the enhanced antioxidative system and others stress responsive genes." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only!
"267-8" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, K., X. Dai, T. Shi, J. Tian, and W. Zhang. 2017. Overexpression of rice microRNA319 improves thermotolerance of perennial ryegrass. Agron. Abr. p. 108215. |
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Last checked: 10/12/2017 |
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