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Web URL(s): | https://scisoc.confex.com/crops/2017am/webprogram/Paper108294.html Last checked: 10/12/2017 |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Elmore, Matthew T.;
White, James F.;
Kingsley, Kathryn L.;
Diehl, Katherine H. D.;
Tuck, Daniel P.;
Verma, Satish K. |
Author Affiliation: | Elmore: Department of Plant Biology, Rutgers University, New Brunswick, NJ; White, Kingsley and Tuck: Plant Biology, Rutgers University, New Brunswick, NJ; Diehl: Rutgers University, New Brunswick, NJ; Verma: Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, India |
Title: | Seed endophytes of smooth crabgrass (Digitaria ischaemum) increase white clover (Trifolium repens) and dandelion (Taraxacum officinale) seedling mortality |
Section: | C05 Turfgrass Science Other records with the "C05 Turfgrass Science" Section
Turf management: Pests poster (includes student competition) Other records with the "Turf management: Pests poster (includes student competition)" Section
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Meeting Info.: | Tampa, Florida: October 22-25, 2017 |
Source: | ASA, CSSA and SSSA International Annual Meetings. 2017, p. 108294. |
Publishing Information: | [Milwaukee, Wisconsin]: [American Society of Agronomy and the Entomological Society of America] |
# of Pages: | 1 |
Keywords: | TIC Keywords: Digitaria ischaemum; Endophytes; Evaluations; Phragmites australis; Seedling vigor; Sterilization; Taraxacum officinale; Trifolium repens
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Abstract/Contents: | "The objective of our research was to determine if smooth crabgrass (Digitaria ischaemum (Schreb.) Schreb. ex Muhl.) seed contains endophytes that antagonize competitor plants. Smooth crabgrass seeds harvested in November 2016 from North Brunswick, NJ were surface sterilized by continuous agitation in a 4% sodium hypochlorite solution for 40 minutes. Seeds were rinsed with sterile water and placed on various media to culture surviving fungi and bacteria. After 4-6 days of incubation at 22±2 °C, outgrowing fungi and bacteria from seeds were isolated. This sterilization and culture process was repeated on three separate occasions. Sixteen bacterial isolates were collected and inoculated onto previously surface disinfected seeds of dandelion (Taraxacum officinale [Taraxacum officinale (L.) Weber ex F.H. Wigg] and white clover (Trifolium repens L.) to evaluate their effects on seedling mortality in Petri dish culture. Certain bacterial isolates resulted in >80% mortality of white clover seedlings at 2 weeks after emergence. These isolates increased mortality of dandelion seedlings as well. A seed endophyte isolated from Phragmites australis seed was included in the experiments as a standard and caused 0% white clover seedling mortality. Mortality of seedlings of non-treated sterilized seed was <5%. Colonization of bacteria into root cells was determined by microscopic observation with ROS staining using diamino benzidine (DAB) and will also be presented." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! "812" |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Elmore, M. T., J. F. White, K. L. Kingsley, K. H. D. Diehl, D. P. Tuck, and S. k. Verma. 2017. Seed endophytes of smooth crabgrass (Digitaria ischaemum) increase white clover (Trifolium repens) and dandelion (Taraxacum officinale) seedling mortality. Agron. Abr. p. 108294. |
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