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Web URL(s): | https://scisoc.confex.com/crops/2017am/webprogram/Paper107710.html Last checked: 10/16/2017 |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Huang, Xin |
Author Affiliation: | China Agricultural University, Beijing, China |
Title: | Transcriptional profiling and identification of heat-responsive genes in perennial ryegrass by RNA-sequencing |
Section: | C05 Turfgrass Science Other records with the "C05 Turfgrass Science" Section
Turf ecology and management (includes student competition) Other records with the "Turf ecology and management (includes student competition)" Section
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Meeting Info.: | Tampa, Florida: October 22-25, 2017 |
Source: | ASA, CSSA and SSSA International Annual Meetings. 2017, p. 107710. |
Publishing Information: | [Milwaukee, Wisconsin]: [American Society of Agronomy and the Entomological Society of America] |
# of Pages: | 1 |
Keywords: | TIC Keywords: Heat stress; Lolium perenne; Ribonucleic acid; Temperature response; Transcription factors; cDNA library
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Abstract/Contents: | "Perennial ryegrass (Lolium perenne) is one of the most widely used forage and turf grasses in the world due to its desirable agronomic qualities. However, as a cool-season perennial grass species, high temperature is a major factor limiting its performance in warmer and transition regions. In this study, a de novo transcriptome was generated using a cDNA library constructed from perennial ryegrass leaves subjected to short-term heat stress treatment. Then the expression profiling and identification of perennial ryegrass heat response genes by digital gene expression (DGE) analyses was performed. The goal of this work was to produce expression profiles of high temperature stress responsive genes in perennial ryegrass leaves and further identify the potentially important candidate genes with altered levels of transcript, such as those genes involved in transcriptional regulation, antioxidant responses, plant hormones & signal transduction, and cellular metabolism. The de novo assembly of perennial ryegrass transcriptome in this study obtained more total and annotated unigenes compared to previously published ones. Many DEGs identified were genes that are known to respond to heat stress in plants, including HSFs, HSPs, and antioxidant related genes. In the meanwhile, we also identified 4 gene candidates mainly involved in C4 carbon fixation, and 1 TOR gene. Their exact roles in plant heat stress response need to dissect further. This study would be important by providing the gene resources for improving heat stress tolerance in both perennial ryegrass and other cool-season perennial grass plants." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! "57-10" |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Huang, X. 2017. Transcriptional profiling and identification of heat-responsive genes in perennial ryegrass by RNA-sequencing. Agron. Abr. p. 107710. |
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