Full TGIF Record # 293396
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DOI:10.1007/s00122-002-1007-0
Web URL(s):https://link.springer.com/article/10.1007/s00122-002-1007-0
    Last checked: 12/07/2017
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https://link.springer.com/content/pdf/10.1007%2Fs00122-002-1007-0.pdf
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Publication Type:
i
Refereed
Author(s):Warnke, S. E.; Barker, R. E.; Brilman, L. A.; Young, W. C. III; Cook, R. L.
Author Affiliation:Warnke: USDA-ARS, Natural Arboretum, Washington, DC; Barker: USDA-ARS, National Forage Seed Production Research Center, Corvallis, OR; Brilman: Seed Research of Oregon, Corvallis, OR; Young and Cook: Crop and Soil Science Department, Oregon State Univ., Corvallis, OR
Title:Inheritance of superoxide dismutase (Sod-1) in a perennial x annual ryegrass cross and its allelic distribution among cultivars
Source:Theoretical and Applied Genetics. Vol. 105, No. 8, December 2002, p. 1146-1150.
Publishing Information:Berlin, Germany: Springer
# of Pages:5
Related Web URL:https://link.springer.com/article/10.1007/s00122-002-1007-0#Abs1
    Last checked: 12/07/2017
    Notes: Abstract only
Keywords:TIC Keywords: Alleles; Genetic analysis; Lolium perenne; Lolium x hybridum; Seed testing; Superoxide dismutase
Abstract/Contents:"Identifying annual ryegrass contamination in perennial ryegrass seed lots has been of major interest in seed-testing laboratories and for seed regulatory agencies in the USA for many years. This study was conducted to characterize a superoxide dismutase locus (Sod-1) and determine its potential to distinguish cultivated ryegrass species. The inheritance of Sod-1 was evaluated in a three-generation annual x perennial ryegrass mapping population and segregation fitted an expected 1:2:1 ratio for a single locus with two alleles. The molecular form of the Sod-1 locus was determined by HO2O2 and KCN inhibitor assays which indicated that the Sod-1, and a second independently segregating Sod-2, locus were both Cu/ZnSod enzymes. The common alleles at the Sod-1 locus were scored in 13 annual and 24 perennial ryegrass cultivars to determine the potential of using this locus for species separation. The Sod-1b allele was homozygous in 98% of perennial ryegrass individuals from 24 cultivars, but those not 100% homozygous for Sod-1b were seed lots with unknown contamination from annual ryegrass. These results indicate that the Sod-1b allele in the homozygous condition is a good indicator of perenniality. All eight annual ryegrass cultivars originating in Europe or Asia had a low frequency of Sod-1b homozygous individuals or none at all. The five cultivars originating in the Western Hemisphere, however, had genotype frequencies for homozygous Sod-1b of up to 56%. The potential of the Sod-1 locus to serve as a test to separate the two growth forms depends on the source of the annual-type contamination."
Language:English
References:34
Note:Pictures, b/w
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Warnke, S. E., R. E. Barker, L. A. Brilman, W. C. III Young, and R. L. Cook. 2002. Inheritance of superoxide dismutase (Sod-1) in a perennial x annual ryegrass cross and its allelic distribution among cultivars. Theor. Appl. Genet. 105(8):p. 1146-1150.
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DOI: 10.1007/s00122-002-1007-0
Web URL(s):
https://link.springer.com/article/10.1007/s00122-002-1007-0
    Last checked: 12/07/2017
    Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007%2Fs00122-002-1007-0.pdf
    Last checked: 12/07/2017
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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