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| DOI: | 10.1007/s10535-018-0788-1 |
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| Web URL(s): | https://link.springer.com/article/10.1007/s10535-018-0788-1
Last checked: 05/23/2018
Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007%2Fs10535-018-0788-1.pdf
Last checked: 05/29/2018
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Tan, P.-H.;
Zhang, L.;
Yin, S.-X.;
Teng, K. |
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| Author Affiliation: | Tan: Turfgrass Research Institute, Beijing Forestry University, Beijing, China and Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China; Zhang and Yin: Turfgrass Research Institute, Beijing Forestry University, Beijing, China; Teng: Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China |
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| Title: | Heterologous expression of a novel Poa pratensis gibberellin 2-oxidase gene, PpGA20x, caused dwarfism, late flowering, and increased chlorophyll accumulation in Arabidopsis |
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| Source: | Biologia Plantarum. Vol. 62, No. 3, September 2018, p. [1-9]. |
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| Publishing Information: | Praha, Czech Republic: Czechoslovak Academy of Science, Institute of Experimental Botany |
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| # of Pages: | 9 |
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| Related Web URL: | https://link.springer.com/article/10.1007/s10535-018-0788-1#Abs1
Last checked: 05/29/2018
Notes: Abstract only
https://static-content.springer.com/esm/art%3A10.1007%2Fs10535-018-0788-1/MediaObjects/10535_2018_788_MOESM1_ESM.pdf
Last checked: 02/05/2019
Notes: Supplemental material |
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| Keywords: | TIC Keywords: Arabidopsis; Chlorophyll content; Dwarf cultivars; Gene expression; Gibberellins; Poa pratensis; Transgenic turfgrasses
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| Abstract/Contents: | "Gibberellin 2-oxidases (GA2oxs) irreversibly convert bioactive gibberellins (GAs) and their immediate precursors into inactive GAs via 2-Β hydroxylation and so regulate gibberellin content in plants. However, to the best of our knowledge, little has been known about the GA2oxs and its function in cool season turfgrass Poa pratensis. In this study, rapid amplification of cDNA end (RACE) was employed to isolate PpGA2ox from P. pratensis. The open reading frame of PpGA2ox was 1 047 bp in length, corresponding to 348 amino acids. PpGA2ox was localized in both nucleus and cytoplasm. The expression of PpGA2ox could be up-regulated by 10 μM gibberellic acid, 5 μM methyl jasmonate, or 10 μM indole-3-acetic acid. In addition, its native promoter could drive GUS expression in both leaf apex and shoot apical region. Moreover, overexpression of PpGA2ox in Arabidopsis led to GA-deficiency leading to dwarf phenotype, delayed flowering time, and increased chlorophyll content. Our study suggests that PpGA2ox could be a candidate gene for breeding new cultivars of P. pratensis" |
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| Language: | English |
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| References: | 26 |
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| Note: | Pictures, color
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tan, P.-H., L. Zhang, S.-X. Yin, and K. Teng. 2018. Heterologous expression of a novel Poa pratensis gibberellin 2-oxidase gene, PpGA20x, caused dwarfism, late flowering, and increased chlorophyll accumulation in Arabidopsis. Biol. Plant. 62(3):p. [1-9]. |
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| DOI: 10.1007/s10535-018-0788-1 |
| Web URL(s):
https://link.springer.com/article/10.1007/s10535-018-0788-1
Last checked: 05/23/2018
Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007%2Fs10535-018-0788-1.pdf
Last checked: 05/29/2018
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: b4899444 |
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