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| Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2018.pdf#page=42
Last checked: 06/20/2018
Requires: PDF Reader
Notes: Item is within a single large file; Abstract only |
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| Publication Type:
| Report |
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| Author(s): | Vines, Phillip L.;
Honig, Josh A.;
Vaiciunas, Jennifer;
Kubik, Christine;
Bushman, B. Shaun;
Weibel, Eric N.;
Meyer, William;
Bonos, Stacy A. |
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| Author Affiliation: | Vines, Honig, Vaiciunas, Kubik, Weibel, Meyer, and Bonos: Department of Plant Biology, Rutgers University; Bushman: USDA-ARS, Logan UT |
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| Title: | A perennial ryegrass (Lolium perenne L.) genetic linkage map constructed with single nucleotide polymorphism markers |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, New Jersey: January 12, 2018 |
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| Source: | Proceedings of the Twenty-Seventh Annual Rutgers Turfgrass Symposium. 2018, p. 41. |
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| Publishing Information: | New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Disease susceptibility; Epichloe festucae; Festuca rubra subsp. rubra; Genetic analysis; Lolium perenne; Low maintenance turf; Sclerotinia homoeocarpa
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| Abstract/Contents: | "Strong creeping red fescue (Festuca rubra subsp. rubra) is a commercially important, low-maintenance turfgrass that is often naturally infected with the fungal endophyte Epichloë festucae. Endophyte-mediated enhanced tolerance to dollar spot disease, caused by the fungal pathogen Sclerotinia homoeocarpa F. T. Bennett, has been observed in the field (Clarke et al., 2006). In a previous quantitative transcriptome study of the E. Festucae-strong creeping red fescue interaction, the second most abundant fungal transcript was found to encode a protein similar to antifungal proteins previously identified in Penicillium and Aspergillus species (Ambrose and Belanger, 2012). The purified protein has been shown to inhibit the growth of S. homoeocarpa in plate assays (Tian et al., 2017). To further study the role of this protein in the endophyte-mediated disease tolerance in planta, we carried out a gene knockout of the E. festucae antifungal protein gene. The conventional fungal gene knockout approach that relies on homologous recombination was unsuccessful, likely because this gene is flanked by repeated sequences. However, we successfully applied CRISPR-Cas9 technology to the non-model fungus E. festucae. The Cas9 endonuclease generated a double-strand break at the target site of the antifungal protein gene, and a mutation was introduced when repairing the break. To date, we have identified one mutant with a single base pair insertion at the Cas9 target site, which introduced an early stop codon, and two mutants with large insertions from the fragments of the transformation vector. Next, the knockout isolate will be reintroduced into strong creeping red fescue to evaluate disease susceptibility of the host plant to dollar spot disease using an established greenhouse inoculation assay." |
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| Language: | English |
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| References: | 0 |
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| See Also: | Original version appears in ASA, CSSA and SSSA International Annual Meetings, 2017, p. 107165, with variant title "A genetic linkage map of perennial ryegrass (Lolium perenne L.) using single nucleotide polymorphism markers", R=290041. R=290041 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Vines, P. L., J. A. Honig, J. Vaiciunas, C. Kubik, B. S. Bushman, E. N. Weibel, et al. 2018. A perennial ryegrass (Lolium perenne L.) genetic linkage map constructed with single nucleotide polymorphism markers. Proc. Rutgers Turfgrass Symp. p. 41. |
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| Web URL(s):
https://turf.rutgers.edu/research/abstracts/symposium2018.pdf#page=42
Last checked: 06/20/2018
Requires: PDF Reader
Notes: Item is within a single large file; Abstract only |
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MSU catalog number: b3696858 |
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