Full TGIF Record # 299640
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DOI:10.1007/s11104-018-3633-6
Web URL(s):https://link.springer.com/article/10.1007/s11104-018-3633-6
    Last checked: 07/24/2018
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https://link.springer.com/content/pdf/10.1007%2Fs11104-018-3633-6.pdf
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Publication Type:
i
Refereed
Author(s):Hsu, Pei-Chun Lisa; O'Callaghan, Maureen; Condron, Leo; Hurst, Mark R. H.
Author Affiliation:Hsu, O'Callaghan, and Hurst: Forage Science, AgResearch Limited, Christchurch, New Zealand and Bio-Protection Research Centre, Lincoln University, Lincoln, New Zealand; Condron: Bio-Protection Research Centre, Lincoln University, Lincoln, New Zealand
Title:Use of a gnotobiotic plant assay for assessing root colonization and mineral phosphate solubilization by Paraburkholderia bryophila Ha185 in association with perennial ryegrass (Lolium perenne L.)
Source:Plant and Soil. Vol. 425, No. 1-2, April 2018, p. 43-55.
# of Pages:13
Publishing Information:Dordrecht, The Netherlands: Kluwer Academic Publishers
Related Web URL:https://link.springer.com/article/10.1007/s11104-018-3633-6#Abs1
    Last checked: 07/31/2018
    Notes: Abstract only
Keywords:TIC Keywords: Fertilizer efficacy; Lolium perenne; Paraburkholderia bryophila ; Phosphates; Rhizosphere; Root analysis; Solubility
Abstract/Contents:"Aims The mechanisms by which rhizosphere bacteria increase the availability of mineral P precipitates for plant use are understudied. However, Paraburkholderia bryophila Ha185 is known to solubilize inorganic phosphate in vitro via a novel process. Therefore, this study aimed to demonstrate P solubilization by Ha185 in association with roots of perennial ryegrass (Lolium perenne L.). Methods We developed a gnotobiotic plant assay to assess P solubilization by Ha185 on ryegrass roots under various nutrient conditions. A green fluorescent protein (GFP)-tagged derivative of Ha185 was used in conjunction with fluorescent microscopy and confocal microscopy to visualize colonization of ryegrass roots. Results Ha185 solubilized mineral P (hydroxyapatite) in association with ryegrass roots and increased ryegrass growth by 20% under P-limited conditions. The GFP-tagged Ha185 strain colonized the rhizoplane and penetrated the primary root of ryegrass, possibly through 'crack entry' at the point of lateral root emergence, but also by entering the epidermal cells via root hairs. Conclusions Ha185 supported ryegrass growth under P-limited conditions, indicating this strain may improve availability of soil P for uptake by ryegrass. Tools developed in this study have broad application in the study of rhizobacteria-plant interactions."
Language:English
References:54
Note:Illustrations
Pictures, color
Graphs
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hsu, P.-C. L., M. O'Callaghan, L. Condron, and M. R. H. Hurst. 2018. Use of a gnotobiotic plant assay for assessing root colonization and mineral phosphate solubilization by Paraburkholderia bryophila Ha185 in association with perennial ryegrass (Lolium perenne L.). Plant Soil. 425(1-2):p. 43-55.
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DOI: 10.1007/s11104-018-3633-6
Web URL(s):
https://link.springer.com/article/10.1007/s11104-018-3633-6
    Last checked: 07/24/2018
    Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007%2Fs11104-018-3633-6.pdf
    Last checked: 07/24/2018
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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