Full TGIF Record # 300305
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Web URL(s):http://www.wsweedscience.org/wp-content/uploads/WSWS_2018_Proceedings-Corrected-1.pdf#page=86
    Last checked: 09/05/2018
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Publication Type:
Author(s):Brunharo, Caio Augusto; Morran, Sarah; Hanson, Brad
Author Affiliation:University of California, Davis, Davis, CA
Title:Mechanism of resistance to glyphosate in annual bluegrass from California perennial crops
Section:General session: Project 5. Basic biology and ecology
Other records with the "General session: Project 5. Basic biology and ecology" Section
Meeting Info.:Garden Grove, California: March 12-15, 2018
Source:Western Society of Weed Science Annual Meeting 2018. Vol. 71, 2018, p. 75.
# of Pages:1
Publishing Information:Westminster, Colorado: Western Society of Weed Science
Keywords:TIC Keywords: Glyphosate; Herbicide resistance; Poa annua control; Polymorphism; Selfing
Abstract/Contents:"Poa annua is a widespread weed species in California, competing directly and indirectly with crops for resources. Recently, poor control of this species with glyphosate was reported in the San Joaquin Valley of California in an almond orchard with a history of repetitive glyphosate use. We hypothesized that the control failures were due to the selection of a glyphosate-resistant population P. annua and generated F4 selfed lines from the suspected R population and an susceptible population collected from the same area to confirm and characterize the resistance. Whole-plant dose-response and shikimate accumulation bioassays were conducted to characterize resistance levels of a suspected-resistant P. annua (R) line compared to a known susceptible line (S). Glyphosate absorption, translocation, and metabolism were assessed to study the involvement of non-target-site-based mechanisms of resistance, whereas EPSPS cloning and sequencing were performed to study the involvement of target-site mechanisms. R exhibited an 18-fold higher glyphosate dose necessary to achieve 50% growth reduction compared to S. Lower levels of shikimate accumulated in R compared to S, corroborating with whole-plant dose-response data. It seems that non-target-site mechanisms are not involved in the resistance to glyphosate in this population of P. annua, as no differences in absorption, translocation and metabolism of glyphosate were observed between the lines. A missense single nucleotide polymorphism was observed in the EPSPS at coding position 106, resulting in a leucine to proline substitution. Interestingly, Poa annua is an allotetraploid and this polymorphism was observed exclusively in P. supina homeologs of P. annua, whereas only wild-type alleles were detected in P. infirma homeologs. Future research will explore the allelic contribution in the mechanism of resistance in P. annua from California."
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Geographic Terms:San Joaquin Valley, California
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Brunharo, C. A., S. Morran, and B. Hanson. 2018. Mechanism of resistance to glyphosate in annual bluegrass from California perennial crops. Proc. West. Soc. Weed Sci. 71:p. 75.
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    Last checked: 09/05/2018
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    Notes: Item is within a single large file
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