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| Web URL(s): | https://scisoc.confex.com/scisoc/2018am/meetingapp.cgi/Paper/111652
Last checked: 11/12/2018
Requires: JavaScript |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Doherty, Joseph;
Crouch, Jo Anne;
Roberts, Joseph |
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| Author Affiliation: | Doherty and Roberts: Plant Science and Landscape Architecture, University of Maryland, College Park, MD; Crouch: Mycology & Nematology Genetic Diversity & Biology Laboratory, USDA-ARS, Beltsville, MD |
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| Title: | Creeping bentgrass associated bacterial communities exhibit taxonomic fluctuations through six months following seedling emergence |
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| Section: | C05 turfgrass science
Other records with the "C05 turfgrass science" Section
Molecular techniques, genetics and plant breeding II: Abiotic and biotic stress oral (includes student competition)
Other records with the "Molecular techniques, genetics and plant breeding II: Abiotic and biotic stress oral (includes student competition)" Section
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| Meeting Info.: | Baltimore, Maryland: November 4-7, 2018 |
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| Source: | ASA, CSSA and SSSA International Annual Meetings. 2018, p. 111652. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Canadian Society of Agronomy] |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Bacteria; Biological control organisms; Disease control; Microbial activity; Seedling emergence
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| Abstract/Contents: | "Biological control agents (BCAs) can be utilized to reduce disease incidence while reducing a turfgrass manager's reliance on conventional fungicides. However, inconsistent efficacy of these BCAs has prevented them from being widely adopted for disease management. By understanding the community dynamics of bacterial populations in the creeping bentgrass microbiome we may elucidate mechanisms to increase establishment and efficacy of bacterial BCAs. To map the bacterial community dynamics in the creeping bentgrass (Agrostis stolonifera L. cv. '007') microbiome, seeds of creeping bentgrass were seeded into replicate cone-tainers filled with a soil medium (85% sand 15% peat, approx. US Golf Assn. rootzone mix) and maintained in a greenhouse. Foliar and rhizosphere samples were taken at emergence, 2, 4 and 6-months post emergence. Amplicons were PCR generated using primers targeting the bacterial 16S v3-v4 hypervariable region and were used to generate next-generation sequencing libraries for processing on an Illumina MiSeq. Sequences were analyzed in R using DADA2, phyloseq and vegan packages. After quality control 8,811 amplicon sequence variants were identified. Ordination analysis and permutational analysis of variance of Bray-Curtis distance matrices revealed significant differences (p<0.05) in community structure between sampling time points and tissue types. Community composition differences were driven by changes in the relative abundances in rare species. Alpha-diversity significantly increased from emergence to 2 months post-emergence. This increase in alpha-diversity coincided with an increase in the number of low relative abundance rarer species present in taxonomic profiling. These data show that turfgrass foliage and rhizosphere host diverse microbial communities. Future research could benefit from focusing on targeting specific microbial groups already present in the microbiome for successful BCA integration." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only!
"71-4" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Doherty, J., J. A. Crouch, and J. Roberts. 2018. Creeping bentgrass associated bacterial communities exhibit taxonomic fluctuations through six months following seedling emergence. Agron. Abr. p. 111652. |
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Last checked: 11/12/2018
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