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Web URL(s): | https://scisoc.confex.com/scisoc/2018am/meetingapp.cgi/Paper/112683 Last checked: 11/12/2018 Requires: JavaScript |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Bernstein, Rachael;
DaCosta, Michelle;
Ebdon, Jeffrey Scott;
Jung, Geunhwa;
Watkins, Eric |
Author Affiliation: | Bernstein: Stockbridge, University of Massachusetts-Amherst, South Hadley, MA; DaCosta: Stockbridge, University of Massachusetts-Amherst, Amherst, MA; Ebdon: Stockbridge School of Agriculture, University of Massachusetts-Amherst, MA; Watkins: Department of Horticultural Science, University of Minnesota, St. Paul, MN |
Title: | Early transcriptome changes associated with loss of freezing tolerance in perennial ryegrass |
Section: | C05 turfgrass science Other records with the "C05 turfgrass science" Section
Molecular techniques, genetics and plant breeding I: cool- and warm- season turf oral Other records with the "Molecular techniques, genetics and plant breeding I: cool- and warm- season turf oral" Section
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Meeting Info.: | Baltimore, Maryland: November 4-7, 2018 |
Source: | ASA, CSSA and SSSA International Annual Meetings. 2018, p. 112683. |
Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Canadian Society of Agronomy] |
# of Pages: | 1 |
Keywords: | TIC Keywords: Cold resistance; Lolium perenne; Ribonucleic acid; Transcription factors
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Abstract/Contents: | "Perennial ryegrass (Lolium perenne L.) is an economically important turf and forage grass, but often exhibits poor winter survival compared to other cool-season grasses. Further research into the mechanisms required for maintenance of freezing tolerance, particularly in response to mild winter temperatures, is needed to help facilitate breeding of cultivars better adapted to northern climates. Previous work in grasses has shown that plants reprogram their transcriptome to mitigate damage caused by prolonged freezing, but less information is known about the regulation of cold deacclimation. The primary objective of this study was to examine early transcriptome changes associated with losses of freezing tolerance during deacclimation. A perennial ryegrass genotype was selected based on sensitivity to deacclimation in previous studies. Plants were exposed to temperature treatments to induce cold acclimation (2°C and -2°C) and deacclimation (8°C for 1 and 3 days). Crowns were harvested following each temperature treatment for RNA-sequencing, allowing for an integrative analysis of differentially expressed genes. At each temperature treatment, plants were harvested for freezing tolerance (LT50) assessment. The LT50 after cold acclimation (2°C and -2°C) was -13.5°C, but significantly increased to -11.8°C during exposure to 8°C for 3 days. Changes in crown transcriptome related to early losses in freezing tolerance will be discussed." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! "28-5" |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Bernstein, R., M. DaCosta, J. S. Ebdon, G. Jung, and E. Watkins. 2018. Early transcriptome changes associated with loss of freezing tolerance in perennial ryegrass. Agron. Abr. p. 112683. |
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