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| Web URL(s): | https://scisoc.confex.com/scisoc/2018am/meetingapp.cgi/Paper/112831
Last checked: 11/13/2018
Requires: JavaScript |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Rushford, Clayton A.;
Miller, Gerald L. |
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| Author Affiliation: | Rushford: University of Missouri-Columbia, Columbia, MO; Miller: Plant Sciences, University of Missouri, Columbia, MO |
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| Title: | Identifying golf course irrigation water as a source of pathogenic Pythium inoculum |
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| Section: | C05 turfgrass science
Other records with the "C05 turfgrass science" Section
Golf turf management II: Soil chemical and physical properties, water, and biotic stress oral (Includes student competition)
Other records with the "Golf turf management II: Soil chemical and physical properties, water, and biotic stress oral (Includes student competition)" Section
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| Meeting Info.: | Baltimore, Maryland: November 4-7, 2018 |
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| Source: | ASA, CSSA and SSSA International Annual Meetings. 2018, p. 112831. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Canadian Society of Agronomy] |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Disease susceptibility; Irrigation water; Pythium
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| Abstract/Contents: | "Creeping bentgrass is often used on golf course putting greens in the US transition zone. Bentgrass is highly susceptible to infection by various Pythium spp. that cause diseases such as Pythium blight, root rot, and root dysfunction. These diseases lower the aesthetic quality of turf and create uneven surfaces that disrupt ball roll. Pathogenic Pythium spp. are known to disseminate through irrigation systems in agricultural and greenhouse settings, but it is unknown if golf course irrigation may serve as a similar inoculum source. Water samples (1.5 L) were collected from irrigation heads surrounding putting greens to screen for the presence of pathogenic Pythium spp. Zoospores were baited from a 500 mL aliquot using sterile creeping bentgrass leaves. Subsequently colonized leaves were cut in half and plated on PARP media for isolation or used directly for DNA extraction. The remaining liter was split in half and filtered using two Durapore filters (5 μm, MilliporeSigma). One filter was plated onto a Pythium specific culture medium and the other was used for direct DNA extraction. Mycelial colonies grown from bentgrass leaves and filters were transferred to fresh V8 media to propagate biomass for DNA extraction. The internal transcribed spacer region of rDNA extracted from mycelia, bait tissue, and membrane filters was PCR-amplified with Oomycete specific primers. Amplicons were sequenced and compared with Genbank accessions using the Basic Local Alignment Search Tool. Pythium was identified from seven out of eleven irrigation sources sampled and two locations produced samples containing two different species. Six Pythium spp. have thus far been identified using these methods. One of these species, P. vanterpoolii, is aggressive and a known causal agent of Pythium root rot. Irrigation water may serve as a source of Pythium inoculum, and management strategies aimed towards reducing this inoculum may aid in disease control." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only!
"49-7" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Rushford, C. A., and G. L. Miller. 2018. Identifying golf course irrigation water as a source of pathogenic Pythium inoculum. Agron. Abr. p. 112831. |
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Last checked: 11/13/2018
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