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| Web URL(s): | https://scisoc.confex.com/scisoc/2018am/meetingapp.cgi/Paper/112269
Last checked: 10/07/2019
Requires: JavaScript |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Buhlman, Jamie;
Harmon, Philip F.;
Milla-Lewis, Susana R.;
Rios, Esteban F.;
Kenworthy, Kevin E. |
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| Author Affiliation: | Buhlman: University of Florida, Gainesville, FL; Harmon: Department of Plant Pathology, University of Florida, Gainesville, FL; Milla-Lewis: Crop & Soil Science, North Carolina State University, Raleigh, NC; Rios: Agronomy, University of Florida, Gainesville, FL; Kenworthy: Agronomy Department, University of Florida, Gainesville, FL |
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| Title: | Analysis of St. Augustinegrass cultivars using SSR markers and testing for the presence of sugarcane mosaic virus |
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| Section: | C05 turfgrass science
Other records with the "C05 turfgrass science" Section
Turf physiology, molecular biology, and genetics poster (Includes student competiton)
Other records with the "Turf physiology, molecular biology, and genetics poster (Includes student competiton)" Section
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| Meeting Info.: | Baltimore, Maryland: November 4-7, 2018 |
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| Source: | ASA, CSSA and SSSA International Annual Meetings. 2018, p. 112269. |
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| Publishing Information: | [Madison, Wisconsin]: [American Society of Agronomy, Crop Science Society of America, and Canadian Society of Agronomy] |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Cultivar evaluation; Disease control; Disease identification; Genetic markers; Simple sequence repeats; Stenotaphrum secundatum; Sugarcane mosaic virus
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| Cultivar Names: | Floratam; Bitterblue |
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| Abstract/Contents: | "St. Augustinegrass (Stenotaphrum secundatum Walt. Kuntze) is the most important turfgrass species for use in landscapes in Florida. 'Floratam' (2n = c. 32) St. Augustinegrass has been in production since the 1970s and accounts for approximately 80% of the St. Augustinegrass sod sold in Florida. An older cultivar, 'Bitterblue' (2n = 3x = 27) has been commercially produced since the early 1900's. The genetic purity of these two widely-grown cultivars, is unknown. It is possible that many fields have contamination and that sod sales of these cultivars are not accurate. Recently, Sugarcane Mosaic Virus (SCMV) has been reported to kill Floratam, making it a potentially devastating disease. However, other St. Augustingrass cultivars are able to survive infection. For this study, sod farms throughout Florida have been asked to voluntarily submit samples of Floratam and Bitterblue St. Augustinegrass. Upon receipt, each sample was tested for the presence of SCMV using a SCMV DAS-ELISA kit, stolon and leaf morphology was recorded and ploidy was analyzed using flow cytometry (FC). Additionally, samples were fingerprinted using a set of twenty-two previously developed simple sequence repeat (SSR) primer pairs. A set of known St. Augustinegrass cultivars were included for comparison when running FC and SSR primer pairs. Primer amplification results were used to verify the cultivar identity of each sample. To date, 12 sod farms have submitted 50 samples of Floratam and eight samples of Bitterblue. Phenotypic evaluations indicate that 17 Floratam samples and two Bitterblue samples are off-types or are contaminated. Results of FC support the phenotypic assessments and FC appears to be a useful tool for initial identification of off-type plants compared to Floratam. This study will assess the status of Floratam and Bitterblue and help growers verify that they are providing SCMV-free sod to consumers." |
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| Language: | English |
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| References: | 0 |
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| See Also: | See also related article "Testing for sugarcane mosaic virus in St. Augustinegrass" Golf Course Management, 87(9) September 2019, p. 69, R=308557. R=308557 |
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| Note: | This item is an abstract only!
"233"
"Poster Number: 1293" |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Buhlman, J., P. F. Harmon, S. R. Milla-Lewis, E. F. Rios, and K. E. Kenworthy. 2018. Analysis of St. Augustinegrass cultivars using SSR markers and testing for the presence of sugarcane mosaic virus. Agron. Abr. p. 112269. |
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Last checked: 10/07/2019
Requires: JavaScript |
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