Full TGIF Record # 30627
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Web URL(s):http://www.tandfonline.com/doi/abs/10.1300/J099v01n01_06#.UqnF--KQO8E
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Publication Type:
i
Report
Author(s):Ha, Sam-Bong; Penmetsa, Ramachandra V.; Wu, Fang-Sheng
Author Affiliation:Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg; Department of Crop and Soil Envrionmental Sciences, Virginia Polytechnic Institute and State University; Department of Biology, Virginia Commonwealth University, Richmond
Title:Transient expression of the Beta-glucuronidase gene driven by different 5' regulatory sequences in tall fescue protoplasts
Source:Journal of Turfgrass Management. Vol. 1, No. 1, 1994, p. 73-81.
# of Pages:9
Publishing Information:Binghamton, NY: Food Products Press (Haworth Press)
Keywords:TIC Keywords: Festuca arundinacea; Genetic engineering; Transgenes; Transgene expression; Promoters; Electroporation
Abstract/Contents:"To identify strongly active 5' regulatory sequences for high levels of transgene expression in tall fescue (Festuca arundinacea Schreb.), we have tested the cauliflower mosaic virus (CaMV) 35S promoter and four different promoter/intron constructs: the CaMV 35S promoter plus the maize alcohol dehydrogenase 1 (adhl) gene intron 1, the CaMV 35S promoter plus the maize adhl intron 6, the rice actin 1 promoter plus its first intron, and the maize adhl promoter plus its first intron. These 5' regulatory sequences were connected to the beta-glucuronidase (GUS) gene for a convenient assay. After the plasmids containing these constructs were introduced into tall fescue protoplasts by electroporation, transient GUS expression was measured to determine their activities. Among the various constructs tested, the rice actin 1 promoter and its first intron showed the highest GUS expression, followed by the CaMV 35S promoter plus maize adhl intron 6. The maize adhl promoter and its first intron showed intermediate GUS expression whereas the CaMV 35S promoter alone or plus the maize adhl intron 1 exhibited very weak GUS expression in tall fescue."
Language:English
References:19
Note:Figures
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ha, S.-B., R. V. Penmetsa, and F.-S. Wu. 1994. Transient expression of the Beta-glucuronidase gene driven by different 5' regulatory sequences in tall fescue protoplasts. J. Turfgrass Manage. 1(1):p. 73-81.
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http://www.tandfonline.com/doi/abs/10.1300/J099v01n01_06#.UqnF--KQO8E
    Last checked: Item not verified
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
    Notes: Abstract and Guide page only
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MSU catalog number: SB 433 .J68
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